Role of the NF-kB/parkin/vegfr-1 pathway associated with hypoxic-ischemic insult in germinal matrix samples of newborn infants.

Autor: Ghirelli EA; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Silva FPGD; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Oricil AGG; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Paula CBV; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Nagashima S; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Oldenburg Neto CF; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Storti E; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Sakiyama FYR; Universidade Federal do Paraná, Curitiba, PR, Brazil., Kayano RM; Universidade Federal do Paraná, Curitiba, PR, Brazil., Sakiyama RR; Universidade Federal do Paraná, Curitiba, PR, Brazil., Moreira VDS; Universidade Federal do Paraná, Curitiba, PR, Brazil., Sotomaior VS; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Noronha L; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.
Jazyk: angličtina
Zdroj: Revista paulista de pediatria : orgao oficial da Sociedade de Pediatria de Sao Paulo [Rev Paul Pediatr] 2023 Apr 28; Vol. 41, pp. e2022034. Date of Electronic Publication: 2023 Apr 28 (Print Publication: 2023).
DOI: 10.1590/1984-0462/2023/41/2022034
Abstrakt: Objective: Given the high proliferative activity of germinal matrix and its direct correlation with hypoxemia, it is necessary to investigate the possible molecular regulation pathways, to understand the existing clinical relationship between the hypoxic-ischemic insult and the biomarkers NF-kB, AKT-3, Parkin, TRK-C and VEGFR-1.
Methods: A hundred and eighteen germinal matrix samples of the central nervous system of patients who died in the first 28 days of life were submitted to histological and immunohistochemistry analysis to identify the tissue immunoexpression of those biomarkers related to asphyxia, prematurity, and death events within 24h.
Results: A significantly increased tissue immunoexpression of NF-kB, AKT-3 and Parkin was observed in the germinal matrix of preterm infants. In addition, significantly decreased tissue immunoexpression of VEGFR-1 and NF-kB was observed in patients who experienced asphyxia followed by death within 24 hours.
Conclusions: The results suggest a direct involvement between the hypoxic-ischemic insult and NF-kB and VEGFR-1 markers since a decreased immunoexpression of these biomarkers was observed in asphyxiated patients. Furthermore, it is suggested that there was not enough time for VEGFR-1 to be transcribed, translated and expressed on the surface of the plasma membrane. This temporality can be observed in the relationship between NF-kB expression and the survival time of individuals who died within 24 hours, suggesting that this factor is essential for the production of VEGFR-1 and, therefore, to carry out the necessary remodeling effect to neovascularize the affected region.
Databáze: MEDLINE
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