Inhibition of angiotensin II type 1 receptor reduces oxidative stress damage to the intestinal barrier in severe acute pancreatitis.

Autor: Gao ZY; Department of the First General Surgery, Qingdao University, Qingdao Hospital, University of Health and Rehabilitation Sciences, Qingdao, China., Jiang YJ; Department of the First General Surgery, Qingdao University, Qingdao Hospital, University of Health and Rehabilitation Sciences, Qingdao, China., Wang J; Department of the First General Surgery, Qingdao University, Qingdao Hospital, University of Health and Rehabilitation Sciences, Qingdao, China., Li C; Department of the First General Surgery, Qingdao University, Qingdao Hospital, University of Health and Rehabilitation Sciences, Qingdao, China., Zhang DL; Department of the First General Surgery, Qingdao University, Qingdao Hospital, University of Health and Rehabilitation Sciences, Qingdao, China.
Jazyk: angličtina
Zdroj: The Kaohsiung journal of medical sciences [Kaohsiung J Med Sci] 2023 Aug; Vol. 39 (8), pp. 824-833. Date of Electronic Publication: 2023 May 03.
DOI: 10.1002/kjm2.12692
Abstrakt: Intestinal barrier injury is a common complication of severe acute pancreatitis (SAP), which is often accompanied by intestinal mucosal barrier injury and results in serious consequences. However, the exact mechanism remains unclear. We aimed to investigate whether angiotensin II type 1 receptor (AT1)-mediated oxidative stress is involved in SAP intestinal barrier injury and assessed the effects of inhibiting this pathway. The SAP model was established by retrograde bile duct injection of sodium taurocholate (5%). The rats were divided into three groups: the control group (SO), the SAP group (SAP), and the azilsartan intervention group (SAP + AZL). Serum amylase, lipase, and other indexes were measured to evaluate SAP severity in each group. Histopathological changes in the pancreas and intestine were evaluated by HE staining. The oxidative stress of intestinal epithelial cells was detected by superoxide dismutase and glutathione. We also detected the expression and distribution of intestinal barrier-related proteins. The results showed that the serum indexes, the severity of tissue damage, and the level of oxidative stress in the SAP + AZL group were significantly lower than in the SAP group. Our study provided hitherto undocumented evidence of AT1 expression in the intestinal mucosa, confirming that AT1-mediated oxidative stress is involved in SAP intestinal mucosal injury, and inhibiting this pathway could effectively reduce intestinal mucosal oxidative stress injury, providing a new and effective target for the treatment of SAP intestinal barrier injury.
(© 2023 The Authors. The Kaohsiung Journal of Medical Sciences published by John Wiley & Sons Australia, Ltd on behalf of Kaohsiung Medical University.)
Databáze: MEDLINE
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