| Autor: |
Conteh JS; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy.; Dipartimento di Chimica e Chimica Industriale, Università di Genova, Via Dodecaneso 31, 16146 Genoa, Italy., Nucci GEP; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy.; Dipartimento di Chimica e Chimica Industriale, Università di Genova, Via Dodecaneso 31, 16146 Genoa, Italy., Fernandez Cabada T; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy., Mai BT; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy., Soni N; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy., De Donato F; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy., Pasquale L; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy., Catalano F; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy., Prato M; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy., Manna L; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy., Pellegrino T; Italian Institute of Technology, via Morego 30, 16163 Genoa, Italy. |
| Abstrakt: |
CuFeS 2 chalcopyrite nanoparticles (NPs) can generate heat under exposure to near-infrared laser irradiation. Here, we develop a protocol to decorate the surface of CuFeS 2 NPs (13 nm) with a thermoresponsive (TR) polymer based on poly(ethylene glycol methacrylate) to combine heat-mediated drug delivery and photothermal heat damage. The resulting TR-CuFeS 2 NPs feature a small hydrodynamic size (∼75 nm), along with high colloidal stability and a TR transition temperature of 41 °C in physiological conditions. Remarkably, TR-CuFeS 2 NPs, when exposed to a laser beam (in the range of 0.5 and 1.5 W/cm 2 ) at NP concentrations as low as 40-50 μg Cu/mL, exhibit a high heating performance with a rise in the solution temperature to hyperthermia therapeutic values (42-45 °C). Furthermore, TR-CuFeS 2 NPs worked as nanocarriers, being able to load an appreciable amount of doxorubicin (90 μg DOXO/mg Cu), a chemotherapeutic agent whose release could then be triggered by exposing the NPs to a laser beam (through which a hyperthermia temperature above 42 °C could be reached). In an in vitro study performed on U87 human glioblastoma cells, bare TR-CuFeS 2 NPs were proven to be nontoxic at a Cu concentration up to 40 μg/mL, while at the same low dose, the drug-loaded TR-CuFeS 2 -DOXO NPs displayed synergistic cytotoxic effects due to the combination of direct heat damage and DOXO chemotherapy, under photo-irradiation by a 808 nm laser (1.2 W/cm 2 ). Finally, under a 808 nm laser, the TR-CuFeS 2 NPs generated a tunable amount of reactive oxygen species depending on the applied power density and NP concentration. |
