Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat production.

Autor:	Stout AJ; Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA., Zhang X; Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA., Letcher SM; Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA., Rittenberg ML; Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.; Biological Engineering Department, Massachusetts Institute of Technology, Cambridge, MA, USA., Shub M; Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA., Chai KM; Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA., Kaul M; Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA., Kaplan DL; Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Apr 17. Date of Electronic Publication: 2023 Apr 17.
DOI:	10.1101/2023.04.17.537163
Abstrakt:	Cultured meat is a promising technology that faces substantial cost barriers which are currently driven largely by the price of media components. Growth factors such as fibroblast growth factor 2 (FGF2) drive the cost of serum-free media for relevant cells including muscle satellite cells. Here, we engineered immortalized bovine satellite cells (iBSCs) for inducible expression of FGF2 and/or mutated Ras G12V in order to overcome media growth factor requirements through autocrine signaling. Engineered cells were able to proliferate over multiple passages in FGF2-free medium, thereby eliminating the need for this costly component. Additionally, cells maintained their myogenicity, albeit with reduced differentiation capacity. Ultimately, this offers a proof-of-principle for lower-cost cultured meat production through cell line engineering. Competing Interests: Declaration of competing interests The authors declare no competing interests.
Databáze:	MEDLINE
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