Finding the right tool: a comprehensive evaluation of microglial inducible cre mouse models.
| Autor: | Bedolla A; Department of Molecular and Cellular Biosciences, University of Cincinnati, Cincinnati, OH 45229, USA.; Neuroscience Graduate Program, University of Cincinnati, Cincinnati, OH 45229, USA., Mckinsey G; Department of Pediatrics, University of California San Francisco, San Francisco, CA 94143, USA.; Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA 94158, USA., Ware K; Department of Molecular and Cellular Biosciences, University of Cincinnati, Cincinnati, OH 45229, USA., Santander N; Instituto de Ciencias de la Salud, Universidad de O´Higgins, Rancagua, Chile., Arnold T; Department of Pediatrics, University of California San Francisco, San Francisco, CA 94143, USA.; Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA 94158, USA., Luo Y; Department of Molecular and Cellular Biosciences, University of Cincinnati, Cincinnati, OH 45229, USA.; Neuroscience Graduate Program, University of Cincinnati, Cincinnati, OH 45229, USA. |
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| Jazyk: | angličtina |
| Zdroj: | BioRxiv : the preprint server for biology [bioRxiv] 2023 Apr 17. Date of Electronic Publication: 2023 Apr 17. |
| DOI: | 10.1101/2023.04.17.536878 |
| Abstrakt: | The recent proliferation of new Cre and CreER recombinase lines provides researchers with a diverse toolkit to study microglial gene function. To determine how best to apply these lines in studies of microglial gene function, a thorough and detailed comparison of their properties is needed. Here, we examined four different microglial CreER lines ( Cx3cr1 CreER(Litt) , Cx3cr1 CreER(Jung) , P2ry12 CreER , Tmem119 CreER ), focusing on (1) recombination specificity; (2) leakiness - degree of non-tamoxifen recombination in microglia and other cells; (3) efficiency of tamoxifen-induced recombination; (4) extra-neural recombination -the degree of recombination in cells outside the CNS, particularly myelo/monocyte lineages (5) off-target effects in the context of neonatal brain development. We identify important caveats and strengths for these lines which will provide broad significance for researchers interested in performing conditional gene deletion in microglia. We also provide data emphasizing the potential of these lines for injury models that result in the recruitment of splenic immune cells. Competing Interests: Declaration of interests The authors declare no competing interests. |
| Databáze: | MEDLINE |
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