Mirror proteorhodopsins.
Autor: | Okhrimenko IS; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Kovalev K; European Molecular Biology Laboratory, Hamburg, Germany., Petrovskaya LE; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia., Ilyinsky NS; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Alekseev AA; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Marin E; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.; Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands., Rokitskaya TI; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Antonenko YN; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Siletsky SA; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Popov PA; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.; iMolecule, Skolkovo Institute of Science and Technology, Moscow, Russia., Zagryadskaya YA; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Soloviov DV; European Molecular Biology Laboratory, Hamburg, Germany., Chizhov IV; Institute for Biophysical Chemistry, Hannover Medical School, Hannover, Germany., Zabelskii DV; European XFEL, Schenefeld, Germany., Ryzhykau YL; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.; Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Russia., Vlasov AV; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.; Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Russia., Kuklin AI; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.; Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Russia., Bogorodskiy AO; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Mikhailov AE; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Sidorov DV; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Bukhalovich S; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Tsybrov F; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Bukhdruker S; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Vlasova AD; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Borshchevskiy VI; Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.; Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Russia., Dolgikh DA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia.; Biological Faculty, Lomonosov Moscow State University, Moscow, Russia., Kirpichnikov MP; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia.; Biological Faculty, Lomonosov Moscow State University, Moscow, Russia., Bamberg E; Max Planck Institute of Biophysics, Frankfurt am Main, Germany., Gordeliy VI; Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CNRS, CEA, Grenoble, France. valentin.gordeliy@ibs.fr. |
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Jazyk: | angličtina |
Zdroj: | Communications chemistry [Commun Chem] 2023 May 02; Vol. 6 (1), pp. 88. Date of Electronic Publication: 2023 May 02. |
DOI: | 10.1038/s42004-023-00884-8 |
Abstrakt: | Proteorhodopsins (PRs), bacterial light-driven outward proton pumps comprise the first discovered and largest family of rhodopsins, they play a significant role in life on the Earth. A big remaining mystery was that up-to-date there was no described bacterial rhodopsins pumping protons at acidic pH despite the fact that bacteria live in different pH environment. Here we describe conceptually new bacterial rhodopsins which are operating as outward proton pumps at acidic pH. A comprehensive function-structure study of a representative of a new clade of proton pumping rhodopsins which we name "mirror proteorhodopsins", from Sphingomonas paucimobilis (SpaR) shows cavity/gate architecture of the proton translocation pathway rather resembling channelrhodopsins than the known rhodopsin proton pumps. Another unique property of mirror proteorhodopsins is that proton pumping is inhibited by a millimolar concentration of zinc. We also show that mirror proteorhodopsins are extensively represented in opportunistic multidrug resistant human pathogens, plant growth-promoting and zinc solubilizing bacteria. They may be of optogenetic interest. (© 2023. The Author(s).) |
Databáze: | MEDLINE |
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