M cell maturation and cDC activation determine the onset of adaptive immune priming in the neonatal Peyer's patch.

Autor:	Torow N; Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany. Electronic address: ntorow@ukaachen.de., Li R; Institute for Computational Genomics, RWTH Aachen University Hospital, Aachen 52074, Germany., Hitch TCA; Functional Microbiome Research Group, Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany., Mingels C; Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany., Al Bounny S; Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany., van Best N; Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany; Department of Medical Microbiology, School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, Maastricht 6200, the Netherlands., Stange EL; Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany., Simons B; Institute of Molecular Medicine, RWTH Aachen University Hospital, Aachen 52074, Germany., Maié T; Institute for Computational Genomics, RWTH Aachen University Hospital, Aachen 52074, Germany., Rüttger L; Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany., Gubbi NMKP; Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany., Abbott DA; Pediatrics Department, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224, USA., Benabid A; Institute for Cell and Tumor Biology, RWTH Aachen University Hospital, Aachen 52074, Germany., Gadermayr M; Institute of Imaging & Computer Vision, RWTH Aachen University, Aachen 52056, Germany., Runge S; Department of Microbiome Research, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen 91054, Germany; Faculty of Biology, University of Freiburg, Freiburg im Breisgau, Germany., Treichel N; Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany., Merhof D; Institute of Imaging & Computer Vision, RWTH Aachen University, Aachen 52056, Germany., Rosshart SP; Department of Microbiome Research, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen 91054, Germany; Department of Medicine II, University of Freiburg, Freiburg im Breisgau, Germany., Jehmlich N; Helmholtz-Centre for Environmental Research GmbH - UFZ, Department of Molecular Systems Biology, Leipzig 04318, Germany., Hand TW; Pediatrics Department, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224, USA., von Bergen M; Helmholtz-Centre for Environmental Research GmbH - UFZ, Department of Molecular Systems Biology, Leipzig 04318, Germany; German Centre for Integrative Biodiversity Research (iDiv), Leipzig 04103, Germany; University of Leipzig, Faculty of Life Sciences, Institute of Biochemistry, Leipzig 04103, Germany., Heymann F; Department of Hepatology & Gastroenterology, Charité University Hospital, Berlin 13353, Germany., Pabst O; Institute of Molecular Medicine, RWTH Aachen University Hospital, Aachen 52074, Germany., Clavel T; Functional Microbiome Research Group, Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany., Tacke F; Department of Hepatology & Gastroenterology, Charité University Hospital, Berlin 13353, Germany., Lelouard H; Aix Marseille University, CNRS, INSERM, CIML, Marseille 13288, France., Costa IG; Institute for Computational Genomics, RWTH Aachen University Hospital, Aachen 52074, Germany., Hornef MW; Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen 52074, Germany. Electronic address: mhornef@ukaachen.de.
Jazyk:	angličtina
Zdroj:	Immunity [Immunity] 2023 Jun 13; Vol. 56 (6), pp. 1220-1238.e7. Date of Electronic Publication: 2023 May 01.
DOI:	10.1016/j.immuni.2023.04.002
Abstrakt:	Early-life immune development is critical to long-term host health. However, the mechanisms that determine the pace of postnatal immune maturation are not fully resolved. Here, we analyzed mononuclear phagocytes (MNPs) in small intestinal Peyer's patches (PPs), the primary inductive site of intestinal immunity. Conventional type 1 and 2 dendritic cells (cDC1 and cDC2) and RORgt+ antigen-presenting cells (RORgt+ APC) exhibited significant age-dependent changes in subset composition, tissue distribution, and reduced cell maturation, subsequently resulting in a lack in CD4+ T cell priming during the postnatal period. Microbial cues contributed but could not fully explain the discrepancies in MNP maturation. Type I interferon (IFN) accelerated MNP maturation but IFN signaling did not represent the physiological stimulus. Instead, follicle-associated epithelium (FAE) M cell differentiation was required and sufficient to drive postweaning PP MNP maturation. Together, our results highlight the role of FAE M cell differentiation and MNP maturation in postnatal immune development. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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