Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells.

Autor:	Lee S; AceBiome Inc., Seoul, Republic of Korea.; R&D Center, AceBiome Inc., Daejeon, Republic of Korea., Lee EJ; AceBiome Inc., Seoul, Republic of Korea.; R&D Center, AceBiome Inc., Daejeon, Republic of Korea., Lee GM; AceBiome Inc., Seoul, Republic of Korea.; R&D Center, AceBiome Inc., Daejeon, Republic of Korea., Yun JH; AceBiome Inc., Seoul, Republic of Korea.; R&D Center, AceBiome Inc., Daejeon, Republic of Korea., Yoo W; AceBiome Inc., Seoul, Republic of Korea.; R&D Center, AceBiome Inc., Daejeon, Republic of Korea.
Jazyk:	angličtina
Zdroj:	Frontiers in nutrition [Front Nutr] 2023 Apr 12; Vol. 10, pp. 1162934. Date of Electronic Publication: 2023 Apr 12 (Print Publication: 2023).
DOI:	10.3389/fnut.2023.1162934
Abstrakt:	Sargassum horneri ( S. horneri ) is a brown seaweed that contains a fucose-rich sulfated polysaccharide called fucoidan and is known to possess beneficial bioactivities, such as anti-inflammatory, antiviral, antioxidative, and antitumoral effects. This study aimed to determine the anti-inflammatory effects of AB_SH (hydrothermal extracts from S. horneri ) and its bioactive compound (fucoidan) against tumor necrosis factor alpha (TNF-α)-induced inflammation in human retinal pigment epithelial (RPE) cells. AB_SH did not exhibit any cytotoxicity, and it decreased the mRNA expression of interleukin (IL)-6 and IL-8 and the production of the cytokines IL-6 and TNF-α. It also suppressed the expression levels of phosphorylated nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs), including c-Jun amino-terminal kinases (JNK), p38 protein kinases (p38), and extracellular signal-regulated kinase (ERK) proteins, suggesting that AB_SH inhibits activation of the NF-kB/MAPK signaling pathway. Since fucoidan was identified in the composition analysis of AB_SH, it was additionally shown to be required for its anti-inflammatory effects in TNF-α-stimulated human RPE cells. In line with the AB_SH results, fucoidan reduced the mRNA levels of IL-6, IL-1ß, and IL-8 and production of the cytokines IL-6, TNF-α, and IL-8 through the downregulation of the NF-kB/MAPK signaling pathway in a dose-dependent manner. Collectively, the ability of AB_SH from S. horneri hydrothermal extracts to reduce inflammation indicates that it may be a good functional ingredient for managing ocular disorders. Competing Interests: SL, EL, GL, J-HY, and WY were employed by AceBiome Inc. (Copyright © 2023 Lee, Lee, Lee, Yun and Yoo.)
Databáze:	MEDLINE
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