Identification of Sutcliffiella horikoshii strains in an immunobiological pharmaceutical industry facility.

Autor:	de Miranda RVDSL; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., da Costa LV; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Albuquerque LS; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Dos Reis CMF; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Braga LMPDS; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., de Andrade JM; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Ramos JN; Interdisciplinary Medical Research Laboratory, IOC/Fiocruz, Brazil., Mattoso JMV; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Forsythe SJ; foodmicrobe.com Ltd, Adams Hill, Keyworth, Nottingham, NG12 5GY, United Kingdom., Brandão MLL; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Jazyk:	angličtina
Zdroj:	Letters in applied microbiology [Lett Appl Microbiol] 2023 May 02; Vol. 76 (5).
DOI:	10.1093/lambio/ovad056
Abstrakt:	The pharmaceutical industry must comply with the requirements for good manufacturing practices to reduce inherent contamination risks in the production process. Bacillus and related genera are among the main bacterial isolated from clean areas, raw material, and products in the pharmaceutical industries, but the correct identification of these species is still a challenge. The aim of this study was to characterize by phenotyping, protein profiling, and 16S rRNA gene sequencing Sutcliffiellahorikoshii strains (n = 6) isolated from an immunobiological pharmaceutical facility, and to propose the reclassification of Bacillus tianshenii to the genus Sutcliffiella, and Sutcliffiella tianshenii sp. nov. The strains were characterized by VITEK®2, matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) using VITEK®MS, and 16S rRNA gene sequencing analysis. MALDI-TOF/MS did not identify any strains that were identified by 16S rRNA as S. horikoshii. VITEK®2 showed false-positive results, with misidentification as B. sporothermodurans (reclassified as Heyndrickxia sporothermodurans) and Geobacillus thermoleovorans. After MALDI-TOF/MS database expansion, with the creation of SuperSpectrum, the strains were correctly identified as S. horikoshii. This study is the first report of isolation of S. horikoshii strains from a pharmaceutical industry. More studies are necessary to better understand the ability of S. horikoshii to contaminate the environment and products. (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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