ALKBH8 as a potential N 6 -methyladenosine (m 6 A) eraser in insects.

Autor: Dai Z; Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, Queensland, Australia., Asgari S; Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, Queensland, Australia.
Jazyk: angličtina
Zdroj: Insect molecular biology [Insect Mol Biol] 2023 Oct; Vol. 32 (5), pp. 461-468. Date of Electronic Publication: 2023 Apr 29.
DOI: 10.1111/imb.12843
Abstrakt: The N 6 -methyladenosine (m 6 A) machinery functions through three groups of proteins in eukaryotic cells, including m 6 A writers, erasers and readers. The m 6 A cellular machinery has mostly been characterised in mammalian species, and the relevant literature on insects is currently scant. While homologues of m 6 A writers and readers have been reported from insects, no erasers have been described so far. Here, using BLAST search, we searched for potential erasers in insects. While we found homologues of human m 6 A eraser ALKBH5 in termites, beetles and true bugs, they could not be found in representative dipteran and lepidopteran species. However, a potential m 6 A eraser, ALKBH8, was identified and experimentally investigated. Our results showed that ALKBH8 can reduce the m 6 A levels of Aedes aegypti and Drosophila melanogaster RNAs, suggesting that AeALKBH8 could be a candidate m 6 A eraser in insects.
(© 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.)
Databáze: MEDLINE
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