Chronic in vivo imaging defines age-dependent alterations of neurogenesis in the mouse hippocampus.

Autor: Wu Y; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Bottes S; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Fisch R; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Zehnder C; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Cole JD; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Pilz GA; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.; BioMedical Center, Department of Cell Biology and Anatomy, Ludwig Maximilians University, Planegg-Martinsried, Germany., Helmchen F; Laboratory of Neural Circuit Dynamics, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Simons BD; Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, UK.; Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.; Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge, Cambridge, UK., Jessberger S; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland. jessberger@hifo.uzh.ch.
Jazyk: angličtina
Zdroj: Nature aging [Nat Aging] 2023 Apr; Vol. 3 (4), pp. 380-390. Date of Electronic Publication: 2023 Feb 20.
DOI: 10.1038/s43587-023-00370-9
Abstrakt: Neural stem cells (NSCs) generate new neurons throughout life in the mammalian hippocampus 1 . Advancing age leads to a decline in neurogenesis, which is associated with impaired cognition 2,3 . The cellular mechanisms causing reduced neurogenesis with advancing age remain largely unknown. We genetically labeled NSCs through conditional recombination driven by the regulatory elements of the stem-cell-expressed gene GLI family zinc finger 1 (Gli1) and used chronic intravital imaging to follow individual NSCs and their daughter cells over months within their hippocampal niche 4,5 . We show that aging affects multiple steps, from cell cycle entry of quiescent NSCs to determination of the number of surviving cells, ultimately causing reduced clonal output of individual NSCs. Thus, we here define the developmental stages that may be targeted to enhance neurogenesis with the aim of maintaining hippocampal plasticity with advancing age.
(© 2023. The Author(s).)
Databáze: MEDLINE
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