Expedited diagnosis of pediatric tuberculosis using Truenat MTB-Rif Dx and GeneXpert MTB/RIF.

Autor: Singh UB; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India. drurvashi@gmail.com., Singh M; Division of Epidemiology and Communicable Diseases, Indian Council of Medical Research, New Delhi, India., Sharma S; Department of Pediatrics, National Institute of TB and Respiratory Diseases, New Delhi, India., Mahajan N; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India., Bala K; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India., Srivastav A; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India., Singh KJ; National Institute of Medical Statistics, ICMR, New Delhi, India., Rao MVV; National Institute of Medical Statistics, ICMR, New Delhi, India., Lodha R; Department of Pediatrics, All India Institute of Medical Sciences, New Delhi, India., Kabra SK; Department of Pediatrics, All India Institute of Medical Sciences, New Delhi, India.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Apr 28; Vol. 13 (1), pp. 6976. Date of Electronic Publication: 2023 Apr 28.
DOI: 10.1038/s41598-023-32810-2
Abstrakt: Rapid, cost-effective, and sensitive diagnostic assays are essential for global tuberculosis (TB) control, especially in high TB burden, resource-limited settings. The current study was designed to evaluate diagnostic accuracy of Truenat MTB-Rif Dx (MolBio) in children less than 18 years of age, with symptoms suggestive of TB. Gastric aspirate, induced sputum, and broncho-alveolar lavage samples were subjected simultaneously to AFB-smear, GeneXpert MTB/RIF, liquid culture (MGIT-960) and Truenat MTB-Rif Dx. The index-test results were evaluated against microbiological reference standards (MRS). Truenat MTB-Rif Dx had a sensitivity of 57.1%, specificity of 92% against MRS. The sensitivity and specificity of the Truenat MTB-RIF Dx compared with liquid culture was 58.7% and 87.5% while GeneXpert MTB/RIF was 56% and 91.4%. The performance of both GeneXpert MTB/RIF and Truenat MTB-Rif Dx are comparable. Result of our study demonstrates that Truenat MTB-Rif can aid in early and efficient diagnosis of TB in children.
(© 2023. The Author(s).)
Databáze: MEDLINE
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