| Autor: |
Alruwad MI; Department of Pharmacognosy, Faculty of Pharmacy Cairo University, Cairo 11562, Egypt., Sabry MM; Department of Pharmacognosy, Faculty of Pharmacy Cairo University, Cairo 11562, Egypt., Gendy AM; Department of Pharmacology and Toxicology, Faculty of Pharmacy, October 6 University, Giza 12585, Egypt., El-Dine RS; Department of Pharmacognosy, Faculty of Pharmacy Cairo University, Cairo 11562, Egypt., El Hefnawy HM; Department of Pharmacognosy, Faculty of Pharmacy Cairo University, Cairo 11562, Egypt. |
| Jazyk: |
angličtina |
| Zdroj: |
Plants (Basel, Switzerland) [Plants (Basel)] 2023 Apr 12; Vol. 12 (8). Date of Electronic Publication: 2023 Apr 12. |
| DOI: |
10.3390/plants12081626 |
| Abstrakt: |
Traditional medicines are a significant source of phytochemicals with potential anticancer effects. Ten Jordanian plants were chosen to be tested for cytotoxicity on human colorectal (HT-29) and breast adenocarcinoma (MCF-7) cell lines. The ethanol extracts were screened for their potential cytotoxic effects using a Sulforhodamine B (SRB) colorimetric assay, using doxorubicin as positive control. Plants extracts exhibiting marked cytotoxic activity were further investigated by qualitative and quantitative phytochemical methods. Total phenolics were quantified using the Folin-Ciocalteu reagent, while flavonoids were quantified using aluminum chloride. The total saponins of the n -butanol fraction were estimated using diosgenin as a standard. The total alkaloids and total terpenoids were also evaluated using the gravimetric method. As results, Senecio leucanthemifolius (IC 50 : 13.84 μg/mL) and Clematis cirrhosa (IC 50 : 13.28 μg/mL) exhibited marked cytotoxic effects on human colorectal adenocarcinoma (HT-29) cell lines. Total phenolics, flavonoids, saponins, alkaloids, and terpenoids found in Senecio leucanthemifolius were (91.82, 14.90, 14.27, 101, and 135.4 mg/g of dry extract), respectively. They were revealed to be (68.18, 7.16, 31.25, 73.6, and 180 mg/g of dry extract) in Clematis cirrhosa , respectively. Senecio leucanthemifolius and Clematis cirrhosa have been found to possess cytotoxicity against colorectal (HT-29). In conclusion, the findings of this study offer a new perspective on Jordanian plant extracts anticancer activity research. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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