| Autor: |
Chng WH; Integrative Sciences and Engineering Programme, NUS Graduate School, National University of Singapore, Singapore 119077, Singapore.; Department of Pharmacy, National University of Singapore, Singapore 117543, Singapore., Muthuramalingam RPK; Department of Pharmacy, National University of Singapore, Singapore 117543, Singapore., Lou CKL; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Nanomedicine Translational Research Programme, Centre for Nanomedicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119609, Singapore., New S; Department of Pharmacy, National University of Singapore, Singapore 117543, Singapore., Neupane YR; Department of Pharmacy, National University of Singapore, Singapore 117543, Singapore.; Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IA 52242, USA., Lee CK; Department of Pharmacy, National University of Singapore, Singapore 117543, Singapore., Altay Benetti A; Department of Pharmacy, National University of Singapore, Singapore 117543, Singapore., Huang C; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Cardiovascular Research Institute, National University Heart Centre, Singapore 119599, Singapore., Thoniyot P; Institute of Sustainability for Chemicals, Energy and Environment (ICES), A*STAR, Singapore 627833, Singapore., Toh WS; Integrative Sciences and Engineering Programme, NUS Graduate School, National University of Singapore, Singapore 119077, Singapore.; Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119288, Singapore.; Tissue Engineering Program, Life Sciences Institute, National University of Singapore, Singapore 117510, Singapore., Wang JW; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.; Nanomedicine Translational Research Programme, Centre for Nanomedicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119609, Singapore.; Cardiovascular Research Institute, National University Heart Centre, Singapore 119599, Singapore.; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119593, Singapore., Pastorin G; Integrative Sciences and Engineering Programme, NUS Graduate School, National University of Singapore, Singapore 119077, Singapore.; Department of Pharmacy, National University of Singapore, Singapore 117543, Singapore. |
| Abstrakt: |
Extracellular vesicles (EVs), which are miniaturised carriers loaded with functional proteins, lipids, and nucleic acid material, are naturally secreted by cells and show intrinsic pharmacological effects in several conditions. As such, they have the potential to be used for the treatment of various human diseases. However, the low isolation yield and laborious purification process are obstacles to their translation for clinical use. To overcome this problem, our lab developed cell-derived nanovesicles (CDNs), which are EV mimetics produced by shearing cells through membrane-fitted spin cups. To evaluate the similarities between EVs and CDNs, we compare the physical properties and biochemical composition of monocytic U937 EVs and U937 CDNs. Besides having similar hydrodynamic diameters, the produced CDNs had proteomic, lipidomic, and miRNA profiles with key communalities compared to those of natural EVs. Further characterisation was conducted to examine if CDNs could exhibit similar pharmacological activities and immunogenicity when administered in vivo. Consistently, CDNs and EVs modulated inflammation and displayed antioxidant activities. EVs and CDNs both did not exert immunogenicity when administered in vivo. Overall, CDNs could serve as a scalable and efficient alternative to EVs for further translation into clinical use. |
