| Autor: |
El-Beltagi HS; Agricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia.; Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt., El-Sayed SM; Department of Biochemistry, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt., Abdelhamid AN; Department of Horticulture, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt., Hassan KM; Department of Horticulture, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt., Elshalakany WA; Department of Biochemistry, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt., Nossier MI; Soil and Water Department, Faculty of Agriculture 11241, Ain Shams University, Cairo 11566, Egypt., Alabdallah NM; Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.; Basic & Applied Scientific Research Centre, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia., Al-Harbi NA; Biology Department, University College of Tayma, University of Tabuk, Tabuk 47512, Saudi Arabia., Al-Qahtani SM; Biology Department, University College of Tayma, University of Tabuk, Tabuk 47512, Saudi Arabia., Darwish DBE; Biology department, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia.; Botany Department, Faculty of Science, Mansoura University, Mansoura 35511, Egypt., Abbas ZK; Biology department, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia., Ibrahim HA; Department of Biochemistry, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt. |
| Abstrakt: |
Catharanthus roseus is a medicinal plant that produces indole alkaloids, which are utilized in anticancer therapy. Vinblastine and vincristine, two commercially important antineoplastic alkaloids, are mostly found in the leaves of Catharanthus roseus . ĸ-carrageenan has been proven as plant growth promoting substance for a number of medicinal and agricultural plants. Considering the importance of ĸ-carrageenan as a promoter of plant growth and phytochemical constituents, especially alkaloids production in Catharanthus roseus , an experiment was carried out to explore the effect of ĸ-carrageenan on the plant growth, phytochemicals content, pigments content, and production of antitumor alkaloids in Catharanthus roseus after planting. Foliar application of ĸ-carrageenan (at 0, 400, 600 and 800 ppm) significantly improved the performance of Catharanthus roseus . Phytochemical analysis involved determining the amount of total phenolics (TP), flavonoids (F), free amino acids (FAA), alkaloids (TAC) and pigments contents by spectrophotometer, minerals by ICP, amino acids, phenolic compounds and alkaloids (Vincamine, Catharanthine, Vincracine (Vincristine), and vinblastine) analysis uses HPLC. The results indicated that all examined ĸ-carrageenan treatments led to a significant ( p ≤ 0.05) increase in growth parameters compared to the untreated plants. Phytochemical examination indicates that the spray of ĸ-carrageenan at 800 mg L -1 increased the yield of alkaloids (Vincamine, Catharanthine and Vincracine (Vincristine)) by 41.85 μg/g DW, total phenolic compounds by 3948.6 μg gallic/g FW, the content of flavonoids 951.3 μg quercetin /g FW and carotenoids content 32.97 mg/g FW as compared to the control. An amount of 400 ppm ĸ-carrageenan treatment gave the best contents of FAA, Chl a, Chl b and anthocyanin. The element content of K, Ca, Cu, Zn and Se increased by treatments. Amino acids constituents and phenolics compounds contents were altered by ĸ-carrageenan. |
