| Autor: |
Vittorakis E; Department of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.; Department of Cell and Molecular Biology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.; Agios Georgios General Hospital of Chania, 73100 Crete, Greece., Vică ML; Department of Cell and Molecular Biology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Zervaki CO; Agios Georgios General Hospital of Chania, 73100 Crete, Greece., Vittorakis E; Agios Georgios General Hospital of Chania, 73100 Crete, Greece., Maraki S; Department of Clinical Microbiology and Microbial Pathogenesis, University Hospital of Heraklion, 70013 Crete, Greece., Mavromanolaki VE; Department of Clinical Microbiology and Microbial Pathogenesis, University Hospital of Heraklion, 70013 Crete, Greece., Schürger ME; Department of Cell and Molecular Biology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Neculicioiu VS; Department of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Papadomanolaki E; Agios Georgios General Hospital of Chania, 73100 Crete, Greece., Sinanis T; Agios Georgios General Hospital of Chania, 73100 Crete, Greece., Giannoulaki G; Agios Georgios General Hospital of Chania, 73100 Crete, Greece., Xydaki E; Agios Georgios General Hospital of Chania, 73100 Crete, Greece., Kastanakis SG; Agios Georgios General Hospital of Chania, 73100 Crete, Greece., Junie LM; Department of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. |
| Abstrakt: |
S. aureus is a pathogenic bacterium that causesinfections. Its virulence is due to surface components, proteins, virulence genes, SCCmec , pvl , agr , and SEs , which are low molecular weight superantigens. SEs are usually encoded by mobile genetic elements, and horizontal gene transfer accounts for their widespread presence in S. aureus . This study analyzed the prevalence of MRSA and MSSA strains of S. aureus in two hospitals in Greece between 2020-2022 and their susceptibility to antibiotics. Specimens collected were tested using the VITEK 2 system and the PCR technique to detect SCCmec types, agr types, pvl genes, and sem and seg genes. Antibiotics from various classes were also tested. This study examined the prevalence and resistance of S. aureus strains in hospitals. It found a high prevalence of MRSA and that the MRSA strains were more resistant to antibiotics. The study also identified the genotypes of the S. aureus isolates and the associated antibiotic resistances. This highlights the need for continued surveillance and effective strategies to combat the spread of MRSA in hospitals. This study examined the prevalence of the pvl gene and its co-occurrence with other genes in S. aureus strains, as well as their antibiotic susceptibility. The results showed that 19.15% of the isolates were pvl -positive and 80.85% were pvl -negative. The pvl gene co-existed with other genes, such as the agr and enterotoxin genes. The results could inform treatment strategies for S. aureus infections. |
