Photoluminescent Scaffolds Based on Natural and Synthetic Biodegradable Polymers for Bioimaging and Tissue Engineering.

Autor: Trifanova EM; Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, 119333 Moscow, Russia., Babayeva G; N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, 115478 Moscow, Russia.; Research Institute of Molecular and Cellular Medicine, RUDN University, 117198 Moscow, Russia., Khvorostina MA; Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, 119333 Moscow, Russia.; Research Centre for Medical Genetics, 115478 Moscow, Russia., Atanova AV; Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, 119333 Moscow, Russia., Nikolaeva ME; Institute of Physics, Technology, and Informational Systems, Moscow State Pedagogical University, 119991 Moscow, Russia., Sochilina AV; Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, 119333 Moscow, Russia.; Institute of Physics, Technology, and Informational Systems, Moscow State Pedagogical University, 119991 Moscow, Russia.; Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, 117997 Moscow, Russia., Khaydukov EV; Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, 119333 Moscow, Russia.; Institute of Physics, Technology, and Informational Systems, Moscow State Pedagogical University, 119991 Moscow, Russia., Popov VK; Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, 119333 Moscow, Russia.
Jazyk: angličtina
Zdroj: Life (Basel, Switzerland) [Life (Basel)] 2023 Mar 24; Vol. 13 (4). Date of Electronic Publication: 2023 Mar 24.
DOI: 10.3390/life13040870
Abstrakt: Non-invasive visualization and monitoring of tissue-engineered structures in a living organism is a challenge. One possible solution to this problem is to use upconversion nanoparticles (UCNPs) as photoluminescent nanomarkers in scaffolds. We synthesized and studied scaffolds based on natural (collagen-COL and hyaluronic acid-HA) and synthetic (polylactic-co-glycolic acids-PLGA) polymers loaded with β-NaYF 4 :Yb 3+ , Er 3+ nanocrystals (21 ± 6 nm). Histomorphological analysis of tissue response to subcutaneous implantation of the polymer scaffolds in BALB/c mice was performed. The inflammatory response of the surrounding tissues was found to be weak for scaffolds based on HA and PLGA and moderate for COL scaffolds. An epi-luminescent imaging system with 975 nm laser excitation was used for in vivo visualization and photoluminescent analysis of implanted scaffolds. We demonstrated that the UCNPs' photoluminescent signal monotonously decreased in all the examined scaffolds, indicating their gradual biodegradation followed by the release of photoluminescent nanoparticles into the surrounding tissues. In general, the data obtained from the photoluminescent analysis correlated satisfactorily with the histomorphological analysis.
Databáze: MEDLINE
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