| Autor: |
de Sousa RPC; Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança 68600-000, Brazil., Campos PSB; Programa de Pós Graduação em Genética e Biologia Molecular, Universidade Federal do Pará,Belém 66075-110, Brazil., Dos Santos MDS; Programa de Pós Graduação em Genética e Biologia Molecular, Universidade Federal do Pará,Belém 66075-110, Brazil., O'Brien PC; Cambridge Resource Centre for Comparative Genomics, Cambridge, CB3 0ES, UK., Ferguson-Smith MA; Cambridge Resource Centre for Comparative Genomics, Cambridge, CB3 0ES, UK., de Oliveira EHC; Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém 66075-110, Brazil.; Instituto Evandro Chagas, Seção de Meio Ambiente, Ananindeua 67030-000, Brazil. |
| Abstrakt: |
Although molecular information for the wood stork ( Mycteria americana ) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversification of M. americana , and provide evolutionary insights based on phylogenetic data of Ciconiidae. For this, we applied both classical and molecular cytogenetic techniques to define the pattern of distribution of heterochromatic blocks and their chromosomal homology with Gallus gallus (GGA). Maximum likelihood analyses and Bayesian inferences (680 bp COI and 1007 bp Cytb genes) were used to determine their phylogenetic relationship with other storks. The results confirmed 2n = 72, and the heterochromatin distribution pattern was restricted to centromeric regions of the chromosomes. FISH experiments identified fusion and fission events involving chromosomes homologous to GGA macrochromosome pairs, some of which were previously found in other species of Ciconiidae, possibly corresponding to synapomorphies for the group. Phylogenetic analyses resulted in a tree that recovered only Ciconinii as a monophyletic group, while Mycteriini and Leptoptlini tribes were configured as paraphyletic clades. In addition, the association between phylogenetic and cytogenetic data corroborates the hypothesis of a reduction in the diploid number throughout the evolution of Ciconiidae. |
