| Autor: |
Macovei I; Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania., Luca SV; Biothermodynamics, TUM School of Life Sciences, Technical University of Munich, D-85354 Freising, Germany., Skalicka-Woźniak K; Department of Natural Products Chemistry, Medical University of Lublin, 20-093 Lublin, Poland., Horhogea CE; Department of Public Health, Ion Ionescu de la Brad University of Life Sciences, 700489 Iasi, Romania., Rimbu CM; Department of Public Health, Ion Ionescu de la Brad University of Life Sciences, 700489 Iasi, Romania., Sacarescu L; Petru Poni Institute of Macromolecular Chemistry, 700487 Iasi, Romania., Vochita G; NIRDBS, Institute of Biological Research Iasi, 700107 Iasi, Romania., Gherghel D; NIRDBS, Institute of Biological Research Iasi, 700107 Iasi, Romania., Ivanescu BL; Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania., Panainte AD; Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania., Nechita C; Marin Dracea National Institute for Research and Development in Forestry, 725100 Campulung Moldovenesc, Romania., Corciova A; Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania., Miron A; Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. |
| Abstrakt: |
In recent years, phytofunctionalized AgNPs have attracted great interest due to their remarkable biological activities. In the present study, AgNPs were synthesized using Abies alba and Pinus sylvestris bark extracts. The chemical profile of these bark extracts was analyzed by LC-HRMS/MS. As a first step, the synthesis parameters (pH, AgNO 3 concentration, ratio of bark extract and AgNO 3 , temperature, and reaction time) were optimized. The synthesized AgNPs were characterized by ATR-FTIR spectroscopy, DLS, SEM, EDX, and TEM. Their antioxidant, cytotoxic, and antibacterial properties were evaluated by the DPPH, ABTS, MTT, and broth microdilution assays, respectively. Abies alba and Pinus sylvestris bark extract-derived AgNPs were well-dispersed, spherical, small (average particle size of 9.92 and 24.49 nm, respectively), stable (zeta potential values of -10.9 and -10.8 mV, respectively), and cytotoxic to A-375 human malignant melanoma cells (IC 50 = 2.40 ± 0.21 and 6.02 ± 0.61 μg/mL, respectively). The phytosynthesized AgNPs also showed antioxidant and antibacterial effects. |
