| Autor: |
Nazarov PA; Moscow Institute of Physics and Technology,141700 Dolgoprudny, Russia.; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia., Khrulnova SA; Moscow Institute of Physics and Technology,141700 Dolgoprudny, Russia.; National Research Center for Hematology, 117198 Moscow, Russia., Kessenikh AG; Moscow Institute of Physics and Technology,141700 Dolgoprudny, Russia.; Laboratory for Microbiology, BIOTECH University, 125080 Moscow, Russia., Novoyatlova US; Moscow Institute of Physics and Technology,141700 Dolgoprudny, Russia.; Laboratory for Microbiology, BIOTECH University, 125080 Moscow, Russia., Kuznetsova SB; Moscow Institute of Physics and Technology,141700 Dolgoprudny, Russia., Bazhenov SV; Moscow Institute of Physics and Technology,141700 Dolgoprudny, Russia.; Laboratory for Microbiology, BIOTECH University, 125080 Moscow, Russia., Sorochkina AI; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia., Karakozova MV; Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia., Manukhov IV; Moscow Institute of Physics and Technology,141700 Dolgoprudny, Russia.; Laboratory for Microbiology, BIOTECH University, 125080 Moscow, Russia. |
| Abstrakt: |
The search for new antibiotics, substances that kill prokaryotic cells and do not kill eukaryotic cells, is an urgent need for modern medicine. Among the most promising are derivatives of triphenylphosphonium, which can protect the infected organs of mammals and heal damaged cells as mitochondria-targeted antioxidants. In addition to the antioxidant action, triphenylphosphonium derivatives exhibit antibacterial activity. It has recently been reported that triphenylphosphonium derivatives cause either cytotoxic effects or inhibition of cellular metabolism at submicromolar concentrations. In this work, we analyzed the MTT data using microscopy and compared them with data on changes in the luminescence of bacteria. We have shown that, at submicromolar concentrations, only metabolism is inhibited, while an increase in alkyltriphenylphosphonium (CnTPP) concentration leads to adhesion alteration. Thus, our data on eukaryotic and prokaryotic cells confirm a decrease in the metabolic activity of cells by CnTPPs but do not confirm a cytocidal effect of TPPs at submicromolar concentrations. This allows us to consider CnTPP as a non-toxic antibacterial drug at low concentrations and a relatively safe vector for delivering other antibacterial substances into bacterial cells. |
