| Autor: |
Lai MY; Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia., Zen LPY; Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia., Abdul Hamid MH; Vector Borne Disease Sector, Ministry of Health, Putrajaya 62590, Malaysia., Jelip J; Vector Borne Disease Sector, Ministry of Health, Putrajaya 62590, Malaysia., Mudin RN; Vector Borne Disease Sector, Ministry of Health, Putrajaya 62590, Malaysia., Ivan VJS; Hospital Kapit, Pathology Unit, Jalan Mamora, Kapit 96800, Malaysia., Francis LNP; Hospital Kapit, Pathology Unit, Jalan Mamora, Kapit 96800, Malaysia., Saihidi I; Hospital Kapit, Pathology Unit, Jalan Mamora, Kapit 96800, Malaysia., Lau YL; Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia. |
| Abstrakt: |
We propose a protocol suitable for point-of-care diagnosis of malaria utilizing a simple and purification-free DNA extraction method with the combination of loop-mediated isothermal amplification assay and lateral flow (LAMP-LF). The multiplex LAMP-LF platform developed here can simultaneously detect Plasmodium knowlesi, P. vivax, P. falciparum, and Plasmodium genus (for P. malariae and P. ovale ). Through the capillary effect, the results can be observed by the red band signal on the test and control lines within 5 min. The developed multiplex LAMP-LF was tested with 86 clinical blood samples on-site at Hospital Kapit, Sarawak, Malaysia. By using microscopy as the reference method, the multiplex LAMP-LF showed 100% sensitivity (95% confidence interval (CI): 91.4 to 100.00%) and 97.8% specificity (95% CI: 88.2% to 99.9%). The high sensitivity and specificity of multiplex LAMP-LF make it ideal for use as a point-of-care diagnostic tool. The simple and purification-free DNA extraction protocol can be employed as an alternative DNA extraction method for malaria diagnosis in resource-limited settings. By combining the simple DNA extraction protocol and multiplex LAMP-LF approach, we aim to develop a simple-to-handle and easy-to-read molecular diagnostic tool for malaria in both laboratory and on-site settings. |
