| Autor: |
Bitencourt ROB; Graduate Program in Veterinary Sciences, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropédica 23890-000, RJ, Brazil., Santos-Mallet JRD; Oswaldo Cruz Foundation, IOC-FIOCRUZ-RJ, Rio de Janeiro 21040-900, RJ, Brazil.; Oswaldo Cruz Foundation, IOC-FIOCRUZ-PI, Teresina 64001-350, PI, Brazil.; Laboratory of Surveillance and Biodiversity in Health, Iguaçu University-UNIG, Nova Iguaçu 28300-000, RJ, Brazil., Lowenberger C; Centre for Cell Biology, Development and Disease, Department of Biological Sciences, Simon Fraser University, Burnaby, BC V5A 1S6, Canada., Ventura A; Department of Animal Biology, Institute of Health and Biological Sciences, Federal Rural University of Rio de Janeiro, Seropédica 23890-000, RJ, Brazil., Gôlo PS; Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropédica 23890-000, RJ, Brazil., Bittencourt VREP; Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropédica 23890-000, RJ, Brazil., Angelo IDC; Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropédica 23890-000, RJ, Brazil. |
| Abstrakt: |
We assessed the effect of the entomopathogenic fungus Metarhizium anisopliae against Aedes aegypti . Conidia of M. anisopliae strains CG 489, CG 153, and IBCB 481 were grown in Adamek medium under different conditions to improve blastospore production. Mosquito larvae were exposed to blastospores or conidia of the three fungal strains at 1 × 10 7 propagules mL -1 . M. anisopliae IBCB 481 and CG 153 reduced larval survival by 100%, whereas CG 489 decreased survival by about 50%. Blastospores of M. anisopliae IBCB 481 had better results in lowering larval survival. M. anisopliae CG 489 and CG 153 reduced larval survival similarly. For histopathology (HP) and scanning electron microscopy (SEM), larvae were exposed to M. anisopliae CG 153 for 24 h or 48 h. SEM confirmed the presence of fungi in the digestive tract, while HP confirmed that propagules reached the hemocoel via the midgut, damaged the peritrophic matrix, caused rupture and atrophy of the intestinal mucosa, caused cytoplasmic disorganization of the enterocytes, and degraded the brush border. Furthermore, we report for the first time the potential of M. anisopliae IBCB 481 to kill Ae. aegypti larvae and methods to improve the production of blastospores. |
