A low-cost device for cryoanesthesia of neonatal rodents.

Autor:	Jamieson BB; State-dependent Neural Processing Laboratory, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK., Cano-Ferrer X; Making STP, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK., Konstantinou G; Making STP, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK., de Launoit E; Sorbonne Université, Paris Brain Institute - ICM, INSERM, CNRS, AP-HP, Hôpital de la Pitié Salpêtrière, Paris, France., Renier N; Sorbonne Université, Paris Brain Institute - ICM, INSERM, CNRS, AP-HP, Hôpital de la Pitié Salpêtrière, Paris, France., Imbert A; Making STP, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK., Kohl J; State-dependent Neural Processing Laboratory, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK.
Jazyk:	angličtina
Zdroj:	HardwareX [HardwareX] 2023 Apr 08; Vol. 14, pp. e00417. Date of Electronic Publication: 2023 Apr 08 (Print Publication: 2023).
DOI:	10.1016/j.ohx.2023.e00417
Abstrakt:	Studying the development of neural circuits in rodent models requires surgical access to the neonatal brain. Since commercially available stereotaxic and anesthetic equipment is designed for use in adults, reliable targeting of brain structures in such young animals can be challenging. Hypothermic cooling (cryoanesthesia) has been used as a preferred anesthesia approach in neonates. This commonly involves submerging neonates in ice, an approach that is poorly controllable. We have developed an affordable, simple to construct device - CryoPup - that allows for fast and robust cryoanesthesia of rodent pups. CryoPup consists of a microcontroller controlling a Peltier element and a heat exchanger. It is capable of both cooling and heating, thereby also functioning as a heating pad during recovery. Importantly, it has been designed for size compatibility with common stereotaxic frames. We validate CryoPup in neonatal mice, demonstrating that it allows for rapid, reliable and safe cryoanesthesia and subsequent recovery. This open-source device will facilitate future studies into the development of neural circuits in the postnatal brain. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Author(s).)
Databáze:	MEDLINE
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