Metabolic Control Analysis of triacylglycerol accumulation in oilseed rape.

Autor: Fell DA; Oxford Brookes University, Oxford, OX3 0BP, UK. Electronic address: dfell@brookes.ac.uk., Taylor DC; National Research Council of Canada 110 Gymnasium Place, Saskatoon, Saskatchewan, S7N 0W9, Canada., Weselake RJ; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G 2P5, Canada., Harwood JL; Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, Wales, UK.
Jazyk: angličtina
Zdroj: Bio Systems [Biosystems] 2023 May; Vol. 227-228, pp. 104905. Date of Electronic Publication: 2023 Apr 24.
DOI: 10.1016/j.biosystems.2023.104905
Abstrakt: The increasing global demand for vegetable oils will only be met if there are significant improvements in the productivity of the major oil crops, such as oilseed rape. Metabolic engineering offers the prospect of further gains in yield beyond that already achieved by breeding and selection but requires guidance as to the changes that need to be made. Metabolic Control Analysis, through measurement and estimation of flux control coefficients, can indicate which enzymes have the most influence on a desired flux. Some experiments have previously reported flux control coefficients for oil accumulation in the seeds of oilseed rape, and others have measured control coefficient distributions for multi-enzyme segments of oil synthesis in seed embryo metabolism measured in vitro. In addition, other reported manipulations of oil accumulation contain results that are exploited further here to calculate previously unknown flux control coefficients. These results are then assembled within a framework that allows an integrated interpretation of the controls on oil accumulation from the assimilation of CO 2 to deposition of oil in the seed. The analysis shows that the control is distributed to an extent that the gains from amplifying any single target are necessarily limited, but there are candidates for joint amplification that are likely to act synergistically to produce much more significant gains.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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