External Quality Assurance Schemes (EQUASs) and Inter-laboratory Comparison Investigations (ICIs) for human biomonitoring of polycyclic aromatic hydrocarbon (PAH) biomarkers in urine as part of the quality assurance programme under HBM4EU.

Autor: Nübler S; Friedrich-Alexander Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Henkestraße 9-11, 91054, Erlangen, Germany., Esteban López M; National Center for Environmental Health, Instituto de Salud Carlos III, Ctra. Majadahonda a Pozuelo Km2,2, 28220, Madrid, Spain., Castaño A; National Center for Environmental Health, Instituto de Salud Carlos III, Ctra. Majadahonda a Pozuelo Km2,2, 28220, Madrid, Spain., Mol HGJ; Wageningen Food Safety Research, Part of Wageningen University and Research, Akkermaalsbos 2, 6708 WB, Wageningen, the Netherlands., Müller J; Friedrich-Alexander Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Henkestraße 9-11, 91054, Erlangen, Germany., Schäfer M; Friedrich-Alexander Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Henkestraße 9-11, 91054, Erlangen, Germany., Haji-Abbas-Zarrabi K; Friedrich-Alexander Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Henkestraße 9-11, 91054, Erlangen, Germany., Hajslova J; University of Chemistry and Technology Prague, Department of Food Analysis and Nutrition (VSCHT), Technicka 5, 16028, Prague, Czech Republic., Pulkrabova J; University of Chemistry and Technology Prague, Department of Food Analysis and Nutrition (VSCHT), Technicka 5, 16028, Prague, Czech Republic., Dvorakova D; University of Chemistry and Technology Prague, Department of Food Analysis and Nutrition (VSCHT), Technicka 5, 16028, Prague, Czech Republic., Urbancova K; University of Chemistry and Technology Prague, Department of Food Analysis and Nutrition (VSCHT), Technicka 5, 16028, Prague, Czech Republic., Koch HM; Institute for Prevention and Occupational Medicine of the German Social Accident Insurance, Institute of the Ruhr-University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany., Antignac JP; Oniris, INRAE, UMR 1329, Laboratoire D'Etude des Résidus et Contaminants Dans les Aliments (LABERCA), F-44307, Nantes, France., Sakhi AK; Norwegian Institute of Public Health, Oslo, Norway., Vorkamp K; Aarhus University, Department of Environmental Science, Frederiksborgvej 399, 4000, Roskilde, Denmark., Burkhardt T; ABF Analytisch-Biologisches Forschungslabor GmbH, Semmelweisstr. 5, 82152, Planegg, Germany., Scherer M; ABF Analytisch-Biologisches Forschungslabor GmbH, Semmelweisstr. 5, 82152, Planegg, Germany., Göen T; Friedrich-Alexander Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Henkestraße 9-11, 91054, Erlangen, Germany. Electronic address: thomas.goeen@fau.de.
Jazyk: angličtina
Zdroj: International journal of hygiene and environmental health [Int J Hyg Environ Health] 2023 May; Vol. 250, pp. 114169. Date of Electronic Publication: 2023 Apr 24.
DOI: 10.1016/j.ijheh.2023.114169
Abstrakt: Polycyclic aromatic hydrocarbons (PAHs) were included as priority substances for human biomonitoring (HBM) in the European Human Biomonitoring Initiative (HBM4EU), which intended to harmonise and advance HBM across Europe. For this project, a specific Quality Assurance and Quality Control (QA/QC) programme applying Inter-laboratory Comparison Investigations (ICIs) and External Quality Assurance Schemes (EQUASs) was developed to ensure the comparability and accuracy of participating analytical laboratories. This paper presents the results of four ICI/EQUAS rounds for the determination of 13 PAH metabolites in urine, i.e. 1-naphthol, 2-naphthol, 1,2-dihydroxynaphthalene, 2-, 3- and 9-hydroxyfluorene, 1-, 2-, 3-, 4- and 9-hydroxyphenanthrene, 1-hydroxypyrene and 3-hydroxybenzo(a)pyrene. However, 4 PAH metabolites could not be evaluated as the analytical capacity of participating laboratories was too low. Across all rounds and biomarkers, 86% of the participants achieved satisfactory results, although low limits of quantification were required to quantify the urinary metabolites at exposure levels of the general population. Using high-performance liquid or gas chromatography coupled with mass spectrometry (HPLC-MS; GC-MS) and isotope dilution for calibration as well as performing an enzymatic deconjugation step proved to be favourable for the accurate determination of PAHs in urine. Finally, the HBM4EU QA/QC programme identified an international network of laboratories providing comparable results in the analysis of urinary PAH biomarkers, although covering all parameters initially selected was still too challenging.
Competing Interests: Declaration of competing interest The authors have no relevant financial or non-financial interests to disclose.
(Copyright © 2023 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: