| Autor: |
Ivkovic T; Laboratory for Molecular Biology and Endocrinology, Vinca Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia., Tepavcevic S, Romic S, Stojiljkovic M, Kostic M, Stanisic J, Koricanac G, Culafic T |
| Jazyk: |
angličtina |
| Zdroj: |
General physiology and biophysics [Gen Physiol Biophys] 2023 May; Vol. 42 (3), pp. 241-250. |
| DOI: |
10.4149/gpb_2023005 |
| Abstrakt: |
Cholecalciferol improves insulin signaling and glucose metabolism in the heart and reduces circulating non-esterified fatty acids. Cholecalciferol effects on the cardiac fatty acid (FA) metabolism and the consequences on calcium handling were examined. Blood lipid profile was determined. Western blot and qRT-PCR were used to examine protein and mRNA expression. Cholecalciferoltreated rats had increased acetyl CoA carboxylase 2 protein expression and decreased expression of malonyl CoA decarboxylase. In addition, the expression of uncoupling protein 3 was elevated. Also, the level of peroxisome proliferator-activated receptor-gamma coactivator in the nucleus of heart cells was increased along with the level of sarcoplasmic/endoplasmic reticulum Ca2+ATPase in the microsomal fraction. In parallel, the L-type calcium channel and ryanodine receptor expression was reduced. In the heart of healthy rats, cholecalciferol affects proteins regulating malonyl CoA availability and intracellular Ca2+ handling proteins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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