Pan-genome analysis of the Burkholderia gladioli PV. Cocovenenans reveal the extent of variation in the toxigenic gene cluster.

Autor: Gong H; School of Life Science, Huizhou University, Huizhou, 516007, China. Electronic address: mygonghao@163.com., Huang X; Huizhou Institute for Food and Drug Control, Huizhou, 516003, China. Electronic address: 49846120@qq.com., Zhu W; Huizhou Institute for Food and Drug Control, Huizhou, 516003, China. Electronic address: 157620896@qq.com., Chen J; Huizhou Institute for Food and Drug Control, Huizhou, 516003, China. Electronic address: 82507436@qq.com., Huang Y; Huizhou Institute for Food and Drug Control, Huizhou, 516003, China. Electronic address: hyd417@163.com., Zhao Z; Huizhou Institute for Food and Drug Control, Huizhou, 516003, China. Electronic address: 75894756@qq.com., Weng J; Huizhou Institute for Food and Drug Control, Huizhou, 516003, China. Electronic address: 83356450@qq.com., Che Y; Huizhou Institute for Food and Drug Control, Huizhou, 516003, China. Electronic address: 70386783@qq.com., Wang J; School of Life Science, Huizhou University, Huizhou, 516007, China. Electronic address: wjy104@163.com., Wang X; Huizhou Institute for Food and Drug Control, Huizhou, 516003, China. Electronic address: 510179132@qq.com.
Jazyk: angličtina
Zdroj: Food microbiology [Food Microbiol] 2023 Aug; Vol. 113, pp. 104249. Date of Electronic Publication: 2023 Mar 02.
DOI: 10.1016/j.fm.2023.104249
Abstrakt: Burkholderia gladioli has been reported as the pathogen responsible for cases of foodborne illness in many countries. The poisonous bongkrekic acid (BA) produced by B. gladioli was linked to a gene cluster absent in non-pathogenic strains. The whole genome sequence of eight bacteria strains, which were screened from the collected 175 raw food and environmental samples, were assembled and analyzed to detect a significant association of 19 protein-coding genes with the pathogenic status. Except for the common BA synthesis-related gene, several other genes, including the toxin-antitoxin genes, were also absent in the non-pathogenic strains. The bacteria strains with the BA gene cluster were found to form a single cluster in the analysis of all B. gladioli genome assemblies for the variants in the gene cluster. Divergence of this cluster was detected in the analysis for both the flanking sequences and those of the whole genome level, which indicates its complex origin. Genome recombination was found to cause a precise sequence deletion in the gene cluster region, which was found to be predominant in the non-pathogenic strains indicating the possible effect of horizontal gene transfer. Our study provided new information and resources for understanding the evolution and divergence of the B. gladioli species.
Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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