| Autor: |
Grachev MA, Hartmann GR, Maximova TG, Mustaev AA, Schäffner AR, Sieber H, Zaychikov EF |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1986 May 12; Vol. 200 (2), pp. 287-90. |
| DOI: |
10.1016/0014-5793(86)81154-1 |
| Abstrakt: |
DNA-dependent RNA polymerase B (II) from wheat germ was modified by incubation with 4-[N-(beta-hydroxyethyl)-N-methyl]benzaldehyde esters of AMP, ADP or ATP, followed by reduction with NaBH4. Reaction of the modified enzyme with [alpha-32P]UTP in the presence of various DNA templates led to a highly selective affinity labelling of the subunit with Mr 140 000 by covalently linked ApU. Labelling was inhibited by 1 microgram/ml alpha-amanitin. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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