Optimization of laboratory diagnosis of heparin-induced thrombocytopenia using HemosIL-AcuStar-HIT-IgG assay.

Autor:	Tucker CM; Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA, US., Rhoades R; Department of Medicine, Division of Hematology, Thomas Jefferson University, Philadelphia, PA, US., Sharma R; Department of Pediatrics, Division of Hematology/Oncology/BMT, Medical College of Wisconsin, Versiti Blood Research Institute, Milwaukee, WI,US., Gong JZ; Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA, US.
Jazyk:	angličtina
Zdroj:	Laboratory medicine [Lab Med] 2024 Jan 06; Vol. 55 (1), pp. 34-39.
DOI:	10.1093/labmed/lmad029
Abstrakt:	Objective: The aim of this study was to determine an optimal cutoff value for the newly available HemosIL-AcuStar-HIT-IgG assay (AcuStar) for the diagnosis of heparin-induced thrombocytopenia (HIT). Method: We evaluated the performance of AcuStar using serotonin release assay (SRA) as the gold standard and incorporated 4T score calculation in a cohort of suspected HIT cases. Statistical analysis was performed to determine optimal cutoff value for the diagnosis of HIT. Result: A diagnosis of HIT can be excluded with a platelet factor 4 (PF4) value of <0.4 U/mL by AcuStar and 4T score in the low-risk category (≤3). All other cases will require confirmation with a functional test. Conclusion: Our study resulted in the implementation of a diagnostic algorithm for laboratory diagnosis of HIT, which incorporates pretest calculation of 4T score and AcuStar as a screening test, with reflex confirmation by SRA. This new algorithm resulted in extended hours of test availability and a more rapid turnaround time in reporting PF4 results. (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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