Inflammation and Dry Eye-like Symptoms as Concomitant Manifestations of Laryngo-Pharyngeal Reflux.

Autor: Bonini S; Ophthalmology Complex Operative Unit, University Campus Bio-Medico, Rome, Italy., Di Zazzo A; Ophthalmology Complex Operative Unit, University Campus Bio-Medico, Rome, Italy., Surico PL; Ophthalmology Complex Operative Unit, University Campus Bio-Medico, Rome, Italy., Balzamino BO; Research and Development Laboratory for Biochemical, Molecular and Cellular Applications in Ophthalmological Sciences; Research Laboratories in Ophthalmology, IRCCS - Fondazione Bietti, Rome, Italy., Luccarelli V; Otorhinolaryngology (ENT) Complex Operative Unit, University Campus Bio-Medico, Rome, Italy., Niutta M; Ophthalmology Complex Operative Unit, University Campus Bio-Medico, Rome, Italy., Coassin M; Ophthalmology Complex Operative Unit, University Campus Bio-Medico, Rome, Italy., Micera A; Research and Development Laboratory for Biochemical, Molecular and Cellular Applications in Ophthalmological Sciences; Research Laboratories in Ophthalmology, IRCCS - Fondazione Bietti, Rome, Italy.
Jazyk: angličtina
Zdroj: Current eye research [Curr Eye Res] 2023 Aug; Vol. 48 (8), pp. 724-730. Date of Electronic Publication: 2023 May 11.
DOI: 10.1080/02713683.2023.2207210
Abstrakt: Purpose: Laryngopharyngeal reflux (LPR) is a common worldwide disease. LPR symptoms may involve distant organs and tissues including the ocular surface with manifestations of a Dry Eye-like disease. We evaluated the concomitant involvement of the ocular surface in patients with LPR. We also defined the clinical signs and the roles of chemical and neuro-inflammatory mediators in the tears of LPR patients.
Methods: Seventy-seven patients with LPR (mean age 65.8 ± 16.8 SD) and 25 healthy controls (mean age 56.5 ± 16.3 SD) were recruited from the otorhinolaryngology unit. Each subject was evaluated for the presence of concomitant ocular surface disease through clinical examination, including the measurement of tear break-up time (TBUT) and the Ocular Surface Disease Index (OSDI) questionnaire. Tears and conjunctival imprints were collected. The presence of pepsin in tears was detected by ELISA. HLA-DR in conjunctival imprints were imaged by immunofluorescence microscopy. RT-PCR quantified conjunctival mRNA transcripts of HLA-DR, IL-8, MUC5AC, NADPH, VIP, and NPY.
Results: Patients with LPR had significantly increased OSDI and reduced TBUT scores compared to control subjects ( p  < 0.05 each). Pepsin was detected in 51% of patient tears while it was not measurable in the controls ( p  < 0.01). Immunoreactivity for HLA-DR in the conjunctival impressions was greater than for the controls with an increased mRNA expression ( p  < 0.05). mRNA transcripts for IL-8, NADPH, and VIP were significantly increased in LPR patients ( p  < 0.05 each), but neither MUC5AC nor NPY was different from controls.
Conclusions: LPR can adversely affect the ocular surface, leading to moderate signs and symptoms of dry eye. This study provides evidence that the presence of pepsin, HLA-DR immunoreactivity, and increased mRNA expression of neuro-inflammatory markers in the tears and conjunctival imprints of LPR patients suggests a potential link between LPR inflammation and ocular surface disease.
Databáze: MEDLINE
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