Investigation of the effects of mir-219-1 gene variants on the development of disease in non-small cell lung cancer patients.

Autor:	Tas SK; University of Health Sciences Turkey, Hamidiye Faculty of Medicine, Department of Immunology, Istanbul, Turkey., Coskunpinar E; University of Health Sciences Turkey, Hamidiye Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey., Yildiz P; University of Health Sciences Turkey, Yedikule Chest Diseases and Chest Surgery Research and Training Hospital, Third Clinic, Istanbul, Turkey., Bayraktaroğlu M; University of Health Sciences Turkey, Yedikule Chest Diseases and Chest Surgery Research and Training Hospital, Third Clinic, Istanbul, Turkey., Kose T; University of Health Sciences Turkey, Hamidiye Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey., Altunkanat D; University of Health Sciences Turkey, Hamidiye Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey., Kirkik D; University of Health Sciences Turkey, Hamidiye Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey., Tukenmez M; Istanbul University, Istanbul Faculty of Medicine, Department of General Surgery, Istanbul, Turkey.
Jazyk:	angličtina
Zdroj:	African health sciences [Afr Health Sci] 2022 Dec; Vol. 22 (4), pp. 37-45.
DOI:	10.4314/ahs.v22i4.6
Abstrakt:	Background: Various variants of the miR-219-1 gene are one of the first genes associated with NSCLC prognosis in the literature. Objectives: We aimed to genotype two different variants of the miR-219-1 gene and to investigate to using of the result as a biomarker in the diagnosis and treatment of NSCLC. Materials and Methods: The patients were chosen according to International NSCLC criteria and genomic DNA was isolated from blood (138 patients and 100 healthy individuals). Then qRT-PCR was applied to determine the rs213210 and rs421446 variants of miR-219-1 gene polymorphisms. Allele and genotype frequencies were compared using Pearson's chi-square and Fisher's exact tests test. Results: We found that TT genotype (p=0,381) in rs213210 compared with CC genotype (p=0,165) and CC genotype (p=0,823) in rs421446 compared with TT genotype (p=0,537) did not show a significantly increased risk of NSCLC. There is no relationship between polymorphisms in miR-219-1 and the outcome of NSCLC. Conclusion: miRNA single nucleotide polymorphisms can be used as genetic biomarkers to predict cancer susceptibility, early diagnosis, and prognosis. Our study has shown that two variants of miR-219-1 were not related to NSCLC in the Turkish population. The reason for this can be differences in ethnicity, regions, and background of population and these differences could lead to various outcomes. Competing Interests: The authors declare that they have no conflict of interest. (© 2022 Tas SK et al.)
Databáze:	MEDLINE
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