Cardiac PDGFRα + interstitial cells generate spontaneous inward currents that contribute to excitability in the heart.

Autor: Zheng H; Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA., Peri L; Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA., Ward GK; Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA., Sanders KM; Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA., Ward SM; Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA.
Jazyk: angličtina
Zdroj: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2023 May; Vol. 37 (5), pp. e22929.
DOI: 10.1096/fj.202201712R
Abstrakt: The cell types and conductance that contribute to normal cardiac functions remain under investigation. We used mice that express an enhanced green fluorescent protein (eGFP)-histone 2B fusion protein driven off the cell-specific endogenous promoter for Pdgfra to investigate the distribution and functional role of PDGFRα + cells in the heart. Cardiac PDGFRα + cells were widely distributed within the endomysium of atria, ventricle, and sino-atrial node (SAN) tissues. PDGFRα + cells formed a discrete network of cells, lying in close apposition to neighboring cardiac myocytes in mouse and Cynomolgus monkey (Macaca fascicularis) hearts. Expression of eGFP in nuclei allowed unequivocal identification of these cells following enzymatic dispersion of muscle tissues. FACS purification of PDGFRα + cells from the SAN and analysis of gene transcripts by qPCR revealed that they were a distinct population of cells that expressed gap junction transcripts, Gja1 and Gjc1. Cardiac PDGFRα + cells generated spontaneous transient inward currents (STICs) and spontaneous transient depolarizations (STDs) that reversed at 0 mV. Reversal potential was maintained when E Cl  = -40 mV. [Na + ] o replacement and FTY720 abolished STICs, suggesting they were due to a non-selective cation conductance (NSCC) carried by TRPM7. PDGFRα + cells also express β 2 -adrenoceptor gene transcripts, Adrb2. Zinterol, a selective β 2 -receptor agonist, increased the amplitude and frequency of STICs, suggesting these cells could contribute to adrenergic regulation of cardiac excitability. PDGFRα + cells in cardiac muscles generate inward currents via an NSCC. STICs generated by these cells may contribute to the integrated membrane potentials of cardiac muscles, possibly affecting the frequency of pacemaker activity.
(© 2023 Federation of American Societies for Experimental Biology.)
Databáze: MEDLINE
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