Activation of Tm-2 2 resistance is mediated by a conserved cysteine essential for tobacco mosaic virus movement.

Autor: Hak H; Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion, Israel., Raanan H; Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion, Israel.; Gilat Research Center, Agricultural Research Organization, Negev, Israel., Schwarz S; Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion, Israel., Sherman Y; Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion, Israel.; The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel., Dinesh-Kumar SP; Department of Plant Biology and Genome Center, College of Biological Sciences, University of California, Davis, California, USA., Spiegelman Z; Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion, Israel.
Jazyk: angličtina
Zdroj: Molecular plant pathology [Mol Plant Pathol] 2023 Aug; Vol. 24 (8), pp. 838-848. Date of Electronic Publication: 2023 Apr 21.
DOI: 10.1111/mpp.13318
Abstrakt: The tomato Tm-2 2 gene was considered to be one of the most durable resistance genes in agriculture, protecting against viruses of the Tobamovirus genus, such as tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV). However, an emerging tobamovirus, tomato brown rugose fruit virus (ToBRFV), has overcome Tm-2 2 , damaging tomato production worldwide. Tm-2 2 encodes a nucleotide-binding leucine-rich repeat (NLR) class immune receptor that recognizes its effector, the tobamovirus movement protein (MP). Previously, we found that ToBRFV MP (MP ToBRFV ) enabled the virus to overcome Tm-2 2 -mediated resistance. Yet, it was unknown how Tm-2 2 remained durable against other tobamoviruses, such as TMV and ToMV, for over 60 years. Here, we show that a conserved cysteine (C68) in the MP of TMV (MP TMV ) plays a dual role in Tm-2 2 activation and viral movement. Substitution of MP ToBRFV amino acid H67 with the corresponding amino acid in MP TMV (C68) activated Tm-2 2 -mediated resistance. However, replacement of C68 in TMV and ToMV disabled the infectivity of both viruses. Phylogenetic and structural prediction analysis revealed that C68 is conserved among all Solanaceae-infecting tobamoviruses except ToBRFV and localizes to a predicted jelly-roll fold common to various MPs. Cell-to-cell and subcellular movement analysis showed that C68 is required for the movement of TMV by regulating the MP interaction with the endoplasmic reticulum and targeting it to plasmodesmata. The dual role of C68 in viral movement and Tm-2 2 immune activation could explain how TMV was unable to overcome this resistance for such a long period.
(© 2023 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje