A simplified protocol for the generation of cortical brain organoids.
| Autor: | Eigenhuis KN; Department of Cell Biology, Erasmus MC, Rotterdam, Netherlands., Somsen HB; Department of Cell Biology, Erasmus MC, Rotterdam, Netherlands., van der Kroeg M; Department of Psychiatry, Erasmus MC, Rotterdam, Netherlands., Smeenk H; Department of Psychiatry, Erasmus MC, Rotterdam, Netherlands., Korporaal AL; Department of Cell Biology, Erasmus MC, Rotterdam, Netherlands., Kushner SA; Department of Psychiatry, Erasmus MC, Rotterdam, Netherlands.; Department of Psychiatry, Columbia University Irving Medical Center, New York, NY, United States., de Vrij FMS; Department of Psychiatry, Erasmus MC, Rotterdam, Netherlands., van den Berg DLC; Department of Cell Biology, Erasmus MC, Rotterdam, Netherlands. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in cellular neuroscience [Front Cell Neurosci] 2023 Apr 04; Vol. 17, pp. 1114420. Date of Electronic Publication: 2023 Apr 04 (Print Publication: 2023). |
| DOI: | 10.3389/fncel.2023.1114420 |
| Abstrakt: | Human brain organoid technology has the potential to generate unprecedented insight into normal and aberrant brain development. It opens up a developmental time window in which the effects of gene or environmental perturbations can be experimentally tested. However, detection sensitivity and correct interpretation of phenotypes are hampered by notable batch-to-batch variability and low reproducibility of cell and regional identities. Here, we describe a detailed, simplified protocol for the robust and reproducible generation of brain organoids with cortical identity from feeder-independent induced pluripotent stem cells (iPSCs). This self-patterning approach minimizes media supplements and handling steps, resulting in cortical brain organoids that can be maintained over prolonged periods and that contain radial glial and intermediate progenitors, deep and upper layer neurons, and astrocytes. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Eigenhuis, Somsen, van der Kroeg, Smeenk, Korporaal, Kushner, de Vrij and van den Berg.) |
| Databáze: | MEDLINE |
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