Autor: |
Surina NM; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia. opera_ghost@inbox.ru., Fedotova IB; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia., Nikolaev GM; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia., Grechenko VV; Pirogov Russian National Research Medical University, Moscow, 117997, Russia., Gankovskaya LV; Pirogov Russian National Research Medical University, Moscow, 117997, Russia., Ogurtsova AD; Pirogov Russian National Research Medical University, Moscow, 117997, Russia., Poletaeva II; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia. ingapoletaeva@mail.ru. |
Jazyk: |
angličtina |
Zdroj: |
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2023 Apr; Vol. 88 (4), pp. 481-490. |
DOI: |
10.1134/S0006297923040041 |
Abstrakt: |
Neuroinflammation plays an important role in epileptogenesis, however, most studies are performed using pharmacological models of epilepsy, while there are only few data available for non-invasive, including genetic, models. The levels of a number of pro-inflammatory cytokines were examined in the Krushinsky-Molodkina (KM) rat strain with high audiogenic epilepsy (AE) proneness (intense tonic seizure fit in response to loud sound) and in the control strain "0" (not predisposed to AE) using multiplex immunofluorescence magnetic assay (MILLIPLEX map Kit). Cytokine levels were determined in the dorsal striatum tissue and in the brain stem. Background levels of IL-1β, IL-6, and TNF-α in the dorsal striatum of the KM rats were significantly lower than in the rats "0" (by 32.31, 27.84, and 38.87%, respectively, p < 0.05, 0.05, and 0.01), whereas no inter-strain differences in the levels of these metabolites were detected in the brain stem in the "background" state. Four hours after sound exposure, the TNF-α level in the dorsal striatum of the KM rats was significantly lower (by 38.34%, p < 0.01) than in the "0" rats. In the KM rats, the dorsal striatal levels of IL-1β and IL-6 were significantly higher after the sound exposure and subsequent seizure fit, compared to the background (35.29 and 50.21% increase, p < 0.05, 0.01, respectively). In the background state the IL-2 level in the KM rats was not detected, whereas after audiogenic seizures its level was 14.01 pg/ml (significant difference, p < 0.01). In the KM rats the brain stem levels of IL-1β and TNF-α after audiogenic seizures were significantly lower than in the background (13.23 and 23.44% decrease, respectively, p < 0.05). In the rats of the "0" strain, the levels of cytokines in the dorsal striatum after the action of sound (which did not induce AE seizures) were not different from those of the background, while in the brain stem of the "0" strain the levels of IL-1β were lower than in the background (40.28%, p < 0.01). Thus, the differences between the background levels of cytokines and those after the action of sound were different in the rats with different proneness to AE. These data suggest involvement of the analyzed cytokines in pathophysiology of the seizure state, namely in AE seizures. |
Databáze: |
MEDLINE |
Externí odkaz: |
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|