Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope.
| Autor: | Shimadzu S; Graduate School of Science, The University of Tokyo, Tokyo, Japan.; Graduate School of Science, Kobe University, Kobe, Japan., Furuya T; Graduate School of Science, Kobe University, Kobe, Japan.; College of Life Sciences, Ritsumeikan University, Kusatsu, Japan., Ozawa Y; Graduate School of Science, The University of Tokyo, Tokyo, Japan., Fukuda H; Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto University of Advanced Science, Kameoka, Japan., Kondo Y; Graduate School of Science, Kobe University, Kobe, Japan. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Quantitative plant biology [Quant Plant Biol] 2022 Jul 18; Vol. 3, pp. e15. Date of Electronic Publication: 2022 Jul 18 (Print Publication: 2022). |
| DOI: | 10.1017/qpb.2022.12 |
| Abstrakt: | Stem cell fates are spatio-temporally regulated during plant development. Time-lapse imaging of fluorescence reporters is the most widely used method for spatio-temporal analysis of biological processes. However, excitation light for imaging fluorescence reporters causes autofluorescence and photobleaching. Unlike fluorescence reporters, luminescence proteins do not require excitation light, and therefore offer an alternative reporter for long-term and quantitative spatio-temporal analysis. We established an imaging system for luciferase, which enabled monitoring cell fate marker dynamics during vascular development in a vascular cell induction system called VISUAL. Single cells expressing the cambium marker, proAtHB8:ELUC , had sharp luminescence peaks at different time points. Furthermore, dual-color luminescence imaging revealed spatio-temporal relationships between cells that differentiated into xylem or phloem, and cells that transitioned from procambium to cambium. This imaging system enables not only the detection of temporal gene expression, but also facilitates monitoring of spatio-temporal dynamics of cell identity transitions at the single cell level. Competing Interests: The authors declare no competing interests. (© The Author(s) 2022.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|