CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host.

Autor: Chase O; Centre for Research in Agricultural Genomics (CRAG, CSIC-IRTA-UAB-UB), 08193, Cerdanyola del Vallès, Barcelona, Spain., Javed A; Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK., Byrne MJ; Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK.; Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, Fermi Ave, Didcot, Oxfordshire, OX11 0DE, UK., Thuenemann EC; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Lomonossoff GP; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Ranson NA; Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK., López-Moya JJ; Centre for Research in Agricultural Genomics (CRAG, CSIC-IRTA-UAB-UB), 08193, Cerdanyola del Vallès, Barcelona, Spain. juanjose.lopez@cragenomica.es.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2023 Apr 19; Vol. 6 (1), pp. 433. Date of Electronic Publication: 2023 Apr 19.
DOI: 10.1038/s42003-023-04799-x
Abstrakt: Sweet potato feathery mottle virus (SPFMV) and Sweet potato mild mottle virus (SPMMV) are members of the genera Potyvirus and Ipomovirus, family Potyviridae, sharing Ipomoea batatas as common host, but transmitted, respectively, by aphids and whiteflies. Virions of family members consist of flexuous rods with multiple copies of a single coat protein (CP) surrounding the RNA genome. Here we report the generation of virus-like particles (VLPs) by transient expression of the CPs of SPFMV and SPMMV in the presence of a replicating RNA in Nicotiana benthamiana. Analysis of the purified VLPs by cryo-electron microscopy, gave structures with resolutions of 2.6 and 3.0 Å, respectively, showing a similar left-handed helical arrangement of 8.8 CP subunits per turn with the C-terminus at the inner surface and a binding pocket for the encapsidated ssRNA. Despite their similar architecture, thermal stability studies reveal that SPMMV VLPs are more stable than those of SPFMV.
(© 2023. The Author(s).)
Databáze: MEDLINE
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