FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry.
| Autor: | Roache-Johnson KH; Yokogawa Fluid Imaging Technologies, Scarborough, ME, USA. kay.johnson@fluidimaging.com., Stephens NR; Yokogawa Fluid Imaging Technologies, Scarborough, ME, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2635, pp. 219-244. |
| DOI: | 10.1007/978-1-0716-3020-4_13 |
| Abstrakt: | This chapter provides a protocol for a detailed evaluation of phytoplankton and nuisance cyanobacteria with the FlowCam 8400 and the FlowCam Cyano. The chapter includes (i) detailed description of the quality control of fluorescent mode of the FlowCam, (ii) detailing methods for discriminating nuisance cyanobacteria using the FlowCam Cyano, how to set up libraries and classification routines for commonly used classification reports, and (iii) detailing methods for viability staining to quantify LIVE versus DEAD phytoplankton using the FlowCam 8400. (© 2023. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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