Minor Actinides Can Replace Essential Lanthanides in Bacterial Life.

Autor: Singer H; Department of Chemistry, Ludwig-Maximilians-University Munich, 81377, München, Germany., Steudtner R; Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V., 01328, Dresden, Germany., Klein AS; Center for Functional Protein Assemblies & TUM School of Natural Sciences, Technical University of Munich, 85748, Garching, Germany., Rulofs C; Center for Functional Protein Assemblies & TUM School of Natural Sciences, Technical University of Munich, 85748, Garching, Germany., Zeymer C; Center for Functional Protein Assemblies & TUM School of Natural Sciences, Technical University of Munich, 85748, Garching, Germany., Drobot B; Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V., 01328, Dresden, Germany., Pol A; Department of Microbiology, Research Institute for Biological and Environmental Sciences, Radboud University Nijmegen, 6525, AJ Nijmegen, The Netherlands., Cecilia Martinez-Gomez N; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA., Op den Camp HJM; Department of Microbiology, Research Institute for Biological and Environmental Sciences, Radboud University Nijmegen, 6525, AJ Nijmegen, The Netherlands., Daumann LJ; Department of Chemistry, Ludwig-Maximilians-University Munich, 81377, München, Germany.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2023 Aug 01; Vol. 62 (31), pp. e202303669. Date of Electronic Publication: 2023 May 10.
DOI: 10.1002/anie.202303669
Abstrakt: Certain f-block elements-the lanthanides-have biological relevance in the context of methylotrophic bacteria. The respective strains incorporate these 4 f elements into the active site of one of their key metabolic enzymes, a lanthanide-dependent methanol dehydrogenase. In this study, we investigated whether actinides, the radioactive 5 f elements, can replace the essential 4 f elements in lanthanide-dependent bacterial metabolism. Growth studies with Methylacidiphilum fumariolicum SolV and the Methylobacterium extorquens AM1 ΔmxaF mutant demonstrate that americium and curium support growth in the absence of lanthanides. Moreover, strain SolV favors these actinides over late lanthanides when presented with a mixture of equal amounts of lanthanides together with americium and curium. Our combined in vivo and in vitro results establish that methylotrophic bacteria can utilize actinides instead of lanthanides to sustain their one-carbon metabolism if they possess the correct size and a +III oxidation state.
(© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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