Genome Assembly and Population Sequencing Reveal Three Populations and Signatures of Insecticide Resistance of Tuta absoluta in Latin America.
| Autor: | Lewald KM; Department of Entomology and Nematology, University of California, Davis., Tabuloc CA; Department of Entomology and Nematology, University of California, Davis., Godfrey KE; Contained Research Facility, University of California, Davis., Arnó J; IRTA, Cabrils, Spain., Perini CR; Department of Phytosanitary Defense, Federal University of Santa Maria, Brazil., Guedes JC; Department of Phytosanitary Defense, Federal University of Santa Maria, Brazil., Chiu JC; Department of Entomology and Nematology, University of California, Davis. |
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| Jazyk: | angličtina |
| Zdroj: | Genome biology and evolution [Genome Biol Evol] 2023 Apr 06; Vol. 15 (4). |
| DOI: | 10.1093/gbe/evad060 |
| Abstrakt: | Tuta absoluta is one of the largest threats to tomato agriculture worldwide. Native to South America, it has rapidly spread throughout Europe, Africa, and Asia over the past two decades. To understand how T. absoluta has been so successful and to improve containment strategies, high-quality genomic resources and an understanding of population history are critical. Here, we describe a highly contiguous annotated genome assembly, as well as a genome-wide population analysis of samples collected across Latin America. The new genome assembly has an L50 of 17 with only 132 contigs. Based on hundreds of thousands of single nucleotide polymorphisms, we detect three major population clusters in Latin America with some evidence of admixture along the Andes Mountain range. Based on coalescent simulations, we find these clusters diverged from each other tens of thousands of generations ago prior to domestication of tomatoes. We further identify several genomic loci with patterns consistent with positive selection and that are related to insecticide resistance, immunity, and metabolism. This data will further future research toward genetic control strategies and inform future containment policies. (© The Author(s) 2023. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.) |
| Databáze: | MEDLINE |
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