Live Stimulated Raman Histology for the Near-Instant Assessment of Central Nervous System Samples.

Autor: Appay R; Assistance Publique des Hopitaux de Marseille (APHM), Centre Hospitalier Universitaire (CHU) Timone, Anatomy and Pathological Anatomy and Neuropathology Departement, 13385 Marseille, France.; Aix-Marseille University, CNRS, INP, Neurophysiopathology Institute, 13385 Marseille, France.; Aix-Marseille University, CNRS, Centrale Marseille, Institut Fresnel, 13397 Marseille, France., Sarri B; Aix-Marseille University, CNRS, Centrale Marseille, Institut Fresnel, 13397 Marseille, France.; Lightcore Technologies, 13002 Marseille, France., Heuke S; Aix-Marseille University, CNRS, Centrale Marseille, Institut Fresnel, 13397 Marseille, France., Boissonneau S; Departement of Neurosurgery, Aix-Marseille University, Assistance Publique des Hopitaux de Marseille (APHM), Centre Hospitalier Universitaire (CHU) Timone, 13385 Marseille, France., Liu C; Lightcore Technologies, 13002 Marseille, France., Dougy E; Assistance Publique des Hopitaux de Marseille (APHM), Biological Resource Center - Tumorbank and Bank of Muscles, 13385 Marseille, France., Daniel L; Assistance Publique des Hopitaux de Marseille (APHM), Centre Hospitalier Universitaire (CHU) Timone, Anatomy and Pathological Anatomy and Neuropathology Departement, 13385 Marseille, France.; Assistance Publique des Hopitaux de Marseille (APHM), Biological Resource Center - Tumorbank and Bank of Muscles, 13385 Marseille, France., Scavarda D; Department of Pediatric Neurosurgery, Assistance Publique des Hopitaux de Marseille (APHM), Centre Hospitalier Universitaire (CHU) Timone, 13385 Marseille, France., Dufour H; Departement of Neurosurgery, Aix-Marseille University, Assistance Publique des Hopitaux de Marseille (APHM), Centre Hospitalier Universitaire (CHU) Timone, 13385 Marseille, France., Figarella-Branger D; Assistance Publique des Hopitaux de Marseille (APHM), Centre Hospitalier Universitaire (CHU) Timone, Anatomy and Pathological Anatomy and Neuropathology Departement, 13385 Marseille, France.; Aix-Marseille University, CNRS, INP, Neurophysiopathology Institute, 13385 Marseille, France., Rigneault H; Aix-Marseille University, CNRS, Centrale Marseille, Institut Fresnel, 13397 Marseille, France.; Lightcore Technologies, 13002 Marseille, France.
Jazyk: angličtina
Zdroj: The journal of physical chemistry. B [J Phys Chem B] 2023 Apr 27; Vol. 127 (16), pp. 3624-3631. Date of Electronic Publication: 2023 Apr 18.
DOI: 10.1021/acs.jpcb.3c01156
Abstrakt: Central nervous system tumors encompass many heterogeneous neoplasms with different outcomes and treatment strategies. The current classification of these tumors is based on molecular parameters in addition to histopathology to define tumor entities. This genomic characterization of tumors is also becoming increasingly essential for physicians to identify targeted therapy options. The deployment of such genomic profiling relies on an efficient surgical sampling. To perform an appropriate tumor resection and a correct sampling of the tumor, the neurosurgeon may request an intraoperative pathological consultation. Stimulated Raman histology (SRH), an emerging nondestructive imaging technology, can address this challenge. SRH allows for a rapid and label-free microscopic examination of unprocessed tissues samples in near-perfect concordance with standard histology. In this study we showed that SRH enabled the near-instant microscopic examination of various central nervous system samples without any tissue processing such as labeling, freezing nor sectioning. Since SRH imaging is a nondestructive approach, we demonstrated that the tissue could be readily recovered after SRH imaging and reintroduced into the conventional pathology workflow including immunohistochemistry and genomic profiling to establish a definitive diagnosis.
Databáze: MEDLINE
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