Actin filaments accumulated in the nucleus remain in the vicinity of condensing chromosomes in the zebrafish early embryo.

Autor:	Oda H; Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan., Sato Y; Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan., Kawashima SA; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan., Fujiwara Y; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan., Pálfy M; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden-01307, Germany., Wu E; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden-01307, Germany.; University of Lausanne, Center for Integrative Genomics, Lausanne 1015, Switzerland., Vastenhouw NL; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden-01307, Germany.; University of Lausanne, Center for Integrative Genomics, Lausanne 1015, Switzerland., Kanai M; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan., Kimura H; Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan.
Jazyk:	angličtina
Zdroj:	Biology open [Biol Open] 2023 May 15; Vol. 12 (5). Date of Electronic Publication: 2023 May 17.
DOI:	10.1242/bio.059783
Abstrakt:	In the cytoplasm, filamentous actin (F-actin) plays a critical role in cell regulation, including cell migration, stress fiber formation, and cytokinesis. Recent studies have shown that actin filaments that form in the nucleus are associated with diverse functions. Here, using live imaging of an F-actin-specific probe, superfolder GFP-tagged utrophin (UtrCH-sfGFP), we demonstrated the dynamics of nuclear actin in zebrafish (Danio rerio) embryos. In early zebrafish embryos up to around the high stage, UtrCH-sfGFP increasingly accumulated in nuclei during the interphase and reached a peak during the prophase. After nuclear envelope breakdown (NEBD), patches of UtrCH-sfGFP remained in the vicinity of condensing chromosomes during the prometaphase to metaphase. When zygotic transcription was inhibited by injecting α-amanitin, the nuclear accumulation of UtrCH-sfGFP was still observed at the sphere and dome stages, suggesting that zygotic transcription may induce a decrease in nuclear F-actin. The accumulation of F-actin in nuclei may contribute to proper mitotic progression of large cells with rapid cell cycles in zebrafish early embryos, by assisting in NEBD, chromosome congression, and/or spindle assembly. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2023. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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