Genetic characterization of the zoonotic parasite Ancylostoma caninum in the central and eastern United States.

Autor:	Quintana TA; Department of Diagnostic Medicine/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan, KS, USA., Johnson WL; Department of Diagnostic Medicine/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan, KS, USA., Ritchie D; Department of Diagnostic Medicine/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan, KS, USA.; Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS, USA., Smith V; Department of Diagnostic Medicine/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan, KS, USA.; Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS, USA., Martin KA; Department of Pathology, Iowa State University College of Veterinary Medicine, Ames, IA, USA., McMahan K; Department of Pathology, Iowa State University College of Veterinary Medicine, Ames, IA, USA., Brewer MT; Department of Pathology, Iowa State University College of Veterinary Medicine, Ames, IA, USA., Jesudoss Chelladurai JRJ; Department of Diagnostic Medicine/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan, KS, USA.; Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS, USA.
Jazyk:	angličtina
Zdroj:	Journal of helminthology [J Helminthol] 2023 Apr 18; Vol. 97, pp. e37. Date of Electronic Publication: 2023 Apr 18.
DOI:	10.1017/S0022149X23000159
Abstrakt:	Ancylostoma caninum is the most common nematode parasite of dogs in the United States. The present study aimed to describe the molecular epidemiology of A. caninum isolates from the central and eastern states of the United States using the partial mitochondrial cytochrome oxidase ( cox1 ) gene and to compare them with those reported globally. We isolated eggs from faecal samples of dogs and characterized each isolate based on cox1 sequences. A total of 60 samples originating from Kansas, Iowa, New York, Florida and Massachusetts were included. 25 haplotypes were identified in the United States dataset with high haplotype diversity (0.904). Sequence data were compared to sequences from other world regions available in GenBank. Global haplotype analysis demonstrated 35 haplotypes with a haplotype diversity of 0.931. Phylogenetic and network analysis provide evidence for the existence of moderate geographical structuring of A. caninum haplotypes. Our results provide an updated summary of A. caninum haplotypes and data for neutral genetic markers with utility for tracking hookworm populations. Sequences have been deposited in GenBank (ON980650-ON980674). Further studies of isolates from other regions are essential to understand the genetic diversity of this parasite.
Databáze:	MEDLINE
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