ARRDC5 expression is conserved in mammalian testes and required for normal sperm morphogenesis.

Autor: Giassetti MI; Center for Reproductive Biology, Washington State University, Pullman, WA, USA.; School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA., Miao D; Center for Reproductive Biology, Washington State University, Pullman, WA, USA.; School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA., Law NC; Center for Reproductive Biology, Washington State University, Pullman, WA, USA.; Department of Animal Sciences, Washington State University, Pullman, WA, USA., Oatley MJ; Center for Reproductive Biology, Washington State University, Pullman, WA, USA.; School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA., Park J; Center for Reproductive Biology, Washington State University, Pullman, WA, USA.; School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA., Robinson LD; Center for Reproductive Biology, Washington State University, Pullman, WA, USA.; School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA., Maddison LA; Center for Reproductive Biology, Washington State University, Pullman, WA, USA., Bernhardt ML; Center for Reproductive Biology, Washington State University, Pullman, WA, USA., Oatley JM; Center for Reproductive Biology, Washington State University, Pullman, WA, USA. joatley@wsu.edu.; School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA. joatley@wsu.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Apr 17; Vol. 14 (1), pp. 2111. Date of Electronic Publication: 2023 Apr 17.
DOI: 10.1038/s41467-023-37735-y
Abstrakt: In sexual reproduction, sperm contribute half the genomic material required for creation of offspring yet core molecular mechanisms essential for their formation are undefined. Here, the α-arrestin molecule arrestin-domain containing 5 (ARRDC5) is identified as an essential regulator of mammalian spermatogenesis. Multispecies testicular tissue transcriptome profiling indicates that expression of Arrdc5 is testis enriched, if not specific, in mice, pigs, cattle, and humans. Knockout of Arrdc5 in mice leads to male specific sterility due to production of low numbers of sperm that are immotile and malformed. Spermiogenesis, the final phase of spermatogenesis when round spermatids transform to spermatozoa, is defective in testes of Arrdc5 deficient mice. Also, epididymal sperm in Arrdc5 knockouts are unable to capacitate and fertilize oocytes. These findings establish ARRDC5 as an essential regulator of mammalian spermatogenesis. Considering the role of arrestin molecules as modulators of cellular signaling and ubiquitination, ARRDC5 is a potential male contraceptive target.
(© 2023. The Author(s).)
Databáze: MEDLINE
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