Metagenomic Deep Sequencing for Orbital Inflammatory Disease.

Autor:	Vagefi MR; Division of Oculofacial Plastic Surgery, Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA., Idowu OO; Division of Oculofacial Plastic Surgery, Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA., Miller A; Division of Oculofacial Plastic Surgery, Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA., Doan T; Division of Oculofacial Plastic Surgery, Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA.; Francis I. Proctor Foundation, University of California, San Francisco, California, USA., Chen C; Francis I. Proctor Foundation, University of California, San Francisco, California, USA., Hinterwirth A; Francis I. Proctor Foundation, University of California, San Francisco, California, USA., Zhong L; Francis I. Proctor Foundation, University of California, San Francisco, California, USA., Ahmad M; Division of Oculofacial Plastic Surgery, Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA., Ashraf DC; Division of Oculofacial Plastic Surgery, Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA., Grob SR; Division of Oculofacial Plastic Surgery, Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA., Kersten RC; Division of Oculofacial Plastic Surgery, Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA., Winn BJ; Division of Oculofacial Plastic Surgery, Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA.; Ophthalmology Section, Surgical Service, San Francisco Veterans Affairs Medical Center, San Francisco, California, USA.
Jazyk:	angličtina
Zdroj:	Ocular immunology and inflammation [Ocul Immunol Inflamm] 2024 Jul; Vol. 32 (5), pp. 718-721. Date of Electronic Publication: 2023 Apr 17.
DOI:	10.1080/09273948.2023.2199061
Abstrakt:	Purpose: Orbital inflammatory disease (OID) is a heterogeneous group of immunologic disorders whose etiology is often non-specific despite routine investigation. In this proof-of-concept study, metagenomic deep sequencing (MDS) is applied to examine host gene expression in two subtypes of OID. Methods: Prospectively collected lacrimal gland tissue from patients with OID was processed for MDS. Differential gene expression analysis was performed to evaluate for host transcriptome signatures. Proof-of-concept comparison was made between histologically confirmed samples of idiopathic dacryoadenitis and IgG4-related disease (IgG4-RD). Results: Twelve genes were identified to be differentially expressed between idiopathic dacryoadenitis and IgG4-RD. Differences in innate humoral immunity gene expression were observed. Several additional genes of interests were also found to be upregulated in idiopathic dacryoadenitis. Conclusions: A unique transcriptome signature was found when comparing idiopathic dacryoadenitis to IgG4-RD. This suggests that MDS can identify differentially expressed genes in OID. Such insight could potentially provide a better understanding of host gene expression and the inflammatory pathways involved in OID.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu