Eucommiae Folium and Active Compounds Protect Against Mitochondrial Dysfunction-Calcium Overload in Epileptic Hippocampal Neurons Through the Hypertrophic Cardiomyopathy Pathway.

Autor: Zhang SN; College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Dong Qing Nan Road, Guian New Area, 550025, People's Republic of China., Li HM; College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Dong Qing Nan Road, Guian New Area, 550025, People's Republic of China., Liu Q; The Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar, 161006, People's Republic of China., Li XZ; College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Dong Qing Nan Road, Guian New Area, 550025, People's Republic of China. xuzhaoli86@yeah.net., Yang WD; College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Dong Qing Nan Road, Guian New Area, 550025, People's Republic of China. yangwude2020@163.com., Zhou Y; College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Dong Qing Nan Road, Guian New Area, 550025, People's Republic of China. yingzhou71@126.com.
Jazyk: angličtina
Zdroj: Neurochemical research [Neurochem Res] 2023 Sep; Vol. 48 (9), pp. 2674-2686. Date of Electronic Publication: 2023 Apr 17.
DOI: 10.1007/s11064-023-03937-5
Abstrakt: Epilepsy is a chronic brain disease and often occurs suddenly for no reason. Eucommiae folium (EF), an edible herb, can be used in the treatment of various kinds of brain diseases in clinic. From the perspective of safety and efficacy, EF is especially suitable for the treatment of chronic brain diseases. With the help of biolabels, this study was aimed to explore the value and feasibility of EF in the treatment of epilepsy. Proteomics and metabolomics were used to explore the biolabels of EF intervention in brain tissues. Bioinformatics was then applied to topologically analyze its neuroprotective effects and mechanisms and material basis based on biolabels, which were validated in an animal model. The biolabel-led research revealed that EF may exert the therapeutic potential to treat brain diseases through the interaction between multiple compounds and multiple targets, among which its therapeutic potential for epilepsy is particularly prominent. In the pentylenetetrazole-induction model, EF and four active compounds (oleamide, catechol, chlorogenic acid, and kaempferol) protected epileptic hippocampal neurons (Nissl and FJB staining) against mitochondrial dysfunction (MYH6, MYL3, and MYBPC3, etc.) and calcium overload (TNNI3, TNNC1, and TNNT2, etc.) through the hypertrophic cardiomyopathy pathway. This study provides new evidence and insights for the neuroprotective effects of EF, in which four active compounds may be potential drug candidates for the treatment of epilepsy.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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