Multicellular, IVT-derived, unmodified human transcriptome for nanopore-direct RNA analysis.

Autor:	McCormick CA; Department of Bioengineering, Northeastern University, Boston, MA, 02115, United States., Akeson S; Department of Bioengineering, Northeastern University, Boston, MA, 02115, United States., Tavakoli S; Department of Bioengineering, Northeastern University, Boston, MA, 02115, United States., Bloch D; Department of Bioengineering, Northeastern University, Boston, MA, 02115, United States., Klink IN; Department of Bioengineering, Northeastern University, Boston, MA, 02115, United States., Jain M; Department of Bioengineering, Northeastern University, Boston, MA, 02115, United States.; Department of Physics, Northeastern University, Boston, MA, 02115, United States., Rouhanifard SH; Department of Bioengineering, Northeastern University, Boston, MA, 02115, United States.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 May 28. Date of Electronic Publication: 2024 May 28.
DOI:	10.1101/2023.04.06.535889
Abstrakt:	Nanopore direct RNA sequencing (DRS) enables measurements of RNA modifications. Modification-free transcripts are a practical and targeted control for DRS, providing a baseline measurement for canonical nucleotides within a matched and biologically derived sequence context. However, these controls can be challenging to generate and carry nanopore-specific nuances that can impact analysis. We produced DRS datasets using modification-free transcripts from in vitro transcription (IVT) of cDNA from six immortalized human cell lines. We characterized variation across cell lines and demonstrated how these may be interpreted. These data will serve as a versatile control and resource to the community for RNA modification analysis of human transcripts. Competing Interests: COMPETING INTEREST STATEMENT The authors declare no competing interests.
Databáze:	MEDLINE
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