Nickel oxide nanoparticles exposure as a risk factor for male infertility: " In vitro " effects on porcine pre-pubertal Sertoli cells.

Autor:	Arato I; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Giovagnoli S; Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy., Di Michele A; Department of Physics and Geology, University of Perugia, Perugia, Italy., Bellucci C; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Lilli C; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Aglietti MC; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Bartolini D; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Gambelunghe A; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Muzi G; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Calvitti M; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Eugeni E; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Gaggia F; Internal Medicine Endocrine and Metabolic Sciences Unit, Santa Maria della Misericordia Hospital of Perugia, Perugia, Italy., Baroni T; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Mancuso F; Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Luca G; Department of Medicine and Surgery, University of Perugia, Perugia, Italy.; International Biotechnological Center for Endocrine, Metabolic and Embryo-Reproductive Translational Research (CIRTEMER), Department of Medicine and Surgery, University of Perugia, Perugia, Italy.; Division of Medical Andrology and Endocrinology of Reproduction, Saint Mary Hospital, Terni, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2023 Mar 30; Vol. 14, pp. 1063916. Date of Electronic Publication: 2023 Mar 30 (Print Publication: 2023).
DOI:	10.3389/fendo.2023.1063916
Abstrakt:	Lately, nickel oxide nanoparticles (NiO NPs) have been employed in different industrial and biomedical fields. Several studies have reported that NiO NPs may affect the development of reproductive organs inducing oxidative stress and, resulting in male infertility. We investigated the in vitro effects of NiO NPs on porcine pre-pubertal Sertoli cells (SCs) which undergone acute (24 h) and chronic (from 1 up to 3 weeks) exposure at two subtoxic doses of NiO NPs of 1 μg/ml and 5 μg/ml. After NiO NPs exposure we performed the following analysis: (a) SCs morphological analysis (Light Microscopy); (b) ROS production and oxidative DNA damage, gene expression of antioxidant enzymes (c) SCs functionality (AMH, inhibin B Real-time PCR analysis and ELISA test); (d) apoptosis (WB analysis); (e) pro-inflammatory cytokines (Real-time PCR analysis), and (f) MAPK kinase signaling pathway (WB analysis). We found that the SCs exposed to both subtoxic doses of NiO NPs didn't sustain substantial morphological changes. NiO NPs exposure, at each concentration, reported a marked increase of intracellular ROS at the third week of treatment and DNA damage at all exposure times. We demonstrated, un up-regulation of SOD and HO-1 gene expression, at both concentrations tested. The both subtoxic doses of NiO NPs detected a down-regulation of AMH and inhibin B gene expression and secreted proteins. Only the 5 μg/ml dose induced the activation of caspase-3 at the third week. At the two subtoxic doses of NiO NPs a clear pro-inflammatory response was resulted in an up-regulation of TNF-α and IL-6 in terms of mRNA. Finally, an increased phosphorylation ratio of p-ERK1/2, p-38 and p-AKT was observed up to the third week, at both concentrations. Our results show the negative impact of subtoxic doses NiO NPs chronic exposure on porcine SCs functionality and viability. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Arato, Giovagnoli, Di Michele, Bellucci, Lilli, Aglietti, Bartolini, Gambelunghe, Muzi, Calvitti, Eugeni, Gaggia, Baroni, Mancuso and Luca.)
Databáze:	MEDLINE
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