A Proterozoic microbial origin of extant cyanide-hydrolyzing enzyme diversity.

Autor:	Schwartz SL; Department of Civil and Environmental Engineering, University of California, Berkeley, Berkeley, CA, United States.; Graduate Program in Microbiology, Massachusetts Institute of Technology, Cambridge, MA, United States.; Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA, United States., Rangel LT; Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA, United States., Payette JG; Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA, United States., Fournier GP; Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Mar 30; Vol. 14, pp. 1130310. Date of Electronic Publication: 2023 Mar 30 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1130310
Abstrakt:	In addition to its role as a toxic environmental contaminant, cyanide has been hypothesized to play a key role in prebiotic chemistry and early biogeochemical evolution. While cyanide-hydrolyzing enzymes have been studied and engineered for bioremediation, the extant diversity of these enzymes remains underexplored. Additionally, the age and evolution of microbial cyanide metabolisms is poorly constrained. Here we provide comprehensive phylogenetic and molecular clock analyses of the distribution and evolution of the Class I nitrilases, thiocyanate hydrolases, and nitrile hydratases. Molecular clock analyses indicate that bacterial cyanide-reducing nitrilases were present by the Paleo- to Mesoproterozoic, and were subsequently horizontally transferred into eukaryotes. These results present a broad diversity of microbial enzymes that could be optimized for cyanide bioremediation. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Schwartz, Rangel, Payette and Fournier.)
Databáze:	MEDLINE
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