Effects of Bipolar Radiofrequency on Collagen Synthesis from Patients with Brachial Ptosis.

Autor:	Cala Uribe LC; Private Practice, Bogotá, Colombia., Perez Pachon ME; Total Definer, Rochester, Minn., Zannin Ferrero A; The Cell Therapy and Metabolism Research Group at Universidad De La Sabana School of Medicine, Bogotá, Colombia., Neri Morales C; The Cell Therapy and Metabolism Research Group at Universidad De La Sabana School of Medicine, Bogotá, Colombia., Silva Gutierrez J; The Cell Therapy and Metabolism Research Group at Universidad De La Sabana School of Medicine, Bogotá, Colombia., Manrique Cruz AD; The Cell Therapy and Metabolism Research Group at Universidad De La Sabana School of Medicine, Bogotá, Colombia., Maza MF; The Cell Therapy and Metabolism Research Group at Universidad De La Sabana School of Medicine, Bogotá, Colombia., Acero Mondragon EJ; PROSEIM Group, Department of Morphophysiology at Universidad de La Sabana, Bogotá, Colombia., Celis Regalado LG; The Cell Therapy and Metabolism Research Group at Universidad De La Sabana School of Medicine, Bogotá, Colombia.
Jazyk:	angličtina
Zdroj:	Plastic and reconstructive surgery. Global open [Plast Reconstr Surg Glob Open] 2023 Apr 13; Vol. 11 (4), pp. e4924. Date of Electronic Publication: 2023 Apr 13 (Print Publication: 2023).
DOI:	10.1097/GOX.0000000000004924
Abstrakt:	Radiofrequency is frequently used for skin rejuvenation, localized fat elimination and cellulite treatment. It prompts the expression of thermal shock proteins that lead to dermal thickening as a result of collagen synthesis. The authors report a histological and clinical analysis of the arm subdermal changes before and after bipolar radiofrequency treatment plus liposuction to determine their benefits for arm contouring. Methods: Inclusion criteria included patients with stage 1, 2a, and 2b brachial ptosis (Duncan classification) and upper limb fat deposits who were considered candidates for third-generation ultrasound-assisted liposculpture plus radiofrequency-assisted lipolysis/skin tightening. Arm subdermal tissue samples (5 mm³) were analyzed before and after the intervention. We used 10% formaldehyde for tissue fixation and stained each sample with hematoxylin/eosin, Masson trichrome, and antibody markers against the cell cycle Ki-67 protein. Results: We analyzed a total of 12 biopsies from six patients who meet the inclusion/exclusion criteria. Histological findings with hematoxylin/eosin revealed hyperplastic and metaplastic changes with focal distribution within the papillary and reticular dermis. Masson trichrome staining showed an increase of the characteristic basophilia of thin type-I and type-III collagen fibers. In contrast, molecular analysis reported an increase in fibroblast activity mediated by the activation of the heat shock protein HSP47. Conclusion: Radiofrequency may be a great alternative to improve skin retraction in patients with mild to moderate brachial dermatochalasis through the activation of HSP47 heat shock protein and the production of type-I and type-III collagen. Competing Interests: Dr. Laura C. Cala Uribe acts as a speaker for INMODE and receives compensation in return. All the other authors declare that they have no financial interest in relation to the content of this article. (Copyright © 2023 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons.)
Databáze:	MEDLINE
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